Don’t Settle for Less
Don’t Settle for Less
Don’t Settle for Less
OVERVIEW DATA CUSTOMIZATION ORDERING & SPECS RESOURCES
OVERVIEW

The NGS Human Core Exome provides the ability to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

Exceptional performance
Exceptional performance
High fidelity double stranded DNA probes with optimized performance
NGS QC of all probes
Uniform enrichment of target regions
Greater flexibility
Greater flexibility
Customization tools allow the rapid and scalable implementation of custom exomes
Modular kit structure enables workflow flexibility for easy integration into existing protocols
Maximized sequencing efficiency
Maximized sequencing efficiency
Increased depth per sample or more samples per run
Lower sequencing costs
EXOME DEFINITION

33 Mb CCDS coverage

99% of ClinVar variants covered

99.3% of targeted regions covered at 20x with 5.3 Gb

The NGS Human Core Exome provides the ability to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

Exceptional performance
Exceptional performance
High fidelity double stranded DNA probes with optimized performance
NGS QC of all probes
Uniform enrichment of target regions
Greater flexibility
Greater flexibility
Customization tools allow the rapid and scalable implementation of custom exomes
Modular kit structure enables workflow flexibility for easy integration into existing protocols
Maximized sequencing efficiency
Maximized sequencing efficiency
Increased depth per sample or more samples per run
Lower sequencing costs
EXOME DEFINITION

33 Mb CCDS coverage

99% of ClinVar variants covered

99.3% of targeted regions covered at 20x with 5.3 Gb

DATA
Uncompromised Performance

Twist Human Core Exome Kit resulted in a higher number of targets covered with 20 or 30 reads compared to leading competitors.

NGS Fixed Panel Human Core Exome 150x Total Coverage Graph
Efficient Read Usage

Twist Bioscience provides higher library complexity than the competitor kits resulting in a lower duplication rate

NGS Fixed Panel Human Core Exome Duplicate Rate Graph
Run more samples with the same budget

The improved coverage uniformity of the Twist Human Core Exome reduces the amount of sequencing required to achieve the desired coverage depth.

NGS Fixed Panel Human Core Exome Coverage Depth Table
Uncompromised Performance

Twist Human Core Exome Kit resulted in a higher number of targets covered with 20 or 30 reads compared to leading competitors.

NGS Fixed Panel Human Core Exome 150x Total Coverage Graph
Efficient Read Usage

Twist Bioscience provides higher library complexity than the competitor kits resulting in a lower duplication rate

NGS Fixed Panel Human Core Exome Duplicate Rate Graph
Run more samples with the same budget

The improved coverage uniformity of the Twist Human Core Exome reduces the amount of sequencing required to achieve the desired coverage depth.

NGS Fixed Panel Human Core Exome Coverage Depth Table
CUSTOMIZATION
Customization of Core Exome Kit Content and Components

The ability to simply add your spike-in panel to the Core Exome creates added differentiation based on your research requirements. Rapid design optimization with a quick production turnaround reduces overall development time for your custom exome.

  • Additional content with high enrichment uniformity 

  • Increased coverage of specific genes covered by Core Exome 

  • Optimization of spike-in ratio by customer

ustomization of Core Exome Kit Content and Components
Customization of Core Exome Kit Content and Components

The ability to simply add your spike-in panel to the Core Exome creates added differentiation based on your research requirements. Rapid design optimization with a quick production turnaround reduces overall development time for your custom exome.

  • Additional content with high enrichment uniformity 

  • Increased coverage of specific genes covered by Core Exome 

  • Optimization of spike-in ratio by customer

ustomization of Core Exome Kit Content and Components
ORDERING & SPECS
Product SKUs
102025 Twist Human Core Exome, 2 Reactions, Kit
102026 Twist Human Core Exome, 12 Reactions, Kit
102027 Twist Human Core Exome, 96 Reactions, Kit
SPECIFICATIONS
Workflow Step Target Enrichment
Format dsDNA
Panel size 33 Mb
Content Human protein coding genes
Design Databases CCDS
Storage Store at -5 to -30°C

The Twist Human Core Exome are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

Product SKUs
102025 Twist Human Core Exome, 2 Reactions, Kit
102026 Twist Human Core Exome, 12 Reactions, Kit
102027 Twist Human Core Exome, 96 Reactions, Kit
SPECIFICATIONS
Workflow Step Target Enrichment
Format dsDNA
Panel size 33 Mb
Content Human protein coding genes
Design Databases CCDS
Storage Store at -5 to -30°C

The Twist Human Core Exome are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

RESOURCES

Exome Sequencing Savings Calculator

Human Core Exome Panel

Number Of Samples

Mean Target Coverage
The number of times a given nucleotide in the genome has been read on average

Sequencing Platform

Cost Per Run

Single Comparison
Full Comparison

Compared To

Twist Saves You
$
on sequencing project costs
Twist Agilent V6
GB Per Exome*
Gb / exome
Fold-80
On Target Rate
Duplicate Rate
Target space
4.0
1.32
98 %
1.6 %
Gb / exome
Fold-80
On Target Rate
Duplicate Rate
Target space
4.0
1.32
98 %
1.6 %
Max Samples Per Run
SEQ Cost Per Sample $ $
SEQ Cost Per Project $ $
*Based on independent evaluations by Johns Hopkins University and CeGaT
Show Calculations

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