Reproducible. Accurate. Safe.
Reproducible. Accurate. Safe.
Reproducible. Accurate. Safe.
OVERVIEW SARS-COV-2 CONTROLS RESPIRATORY VIRUS CONTROLS RESOURCES
Overview

Trusted, accurate and safe controls for
a wide-range of applications

The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing. 

 

Reproducible, Quality Results

Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays. 

 

A Safe and Effective Alternative to “Live Virus”

Controls based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.

 

Flexible formats for your applications

Choose from Standard, Assay Ready, or Encapsuled Control formulations. The standard format provides the controls at high concentration in a frozen liquid formulation. The Assay Ready Controls are provided as a dried pellet that increases stability. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s The Encapsulated Control products leverage a metal capsule sealed around a desiccated pellet to enhance stability of the RNA controls.
 
Both the Assay Ready and the Encapsulated formulations are single use controls. The Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers. The enhanced stability of the Encapsulated controls enables extended shelf life and both ship and store at room temperature. Ambient shipping reduces cost and increases accessibility for researchers across the globe. 

 

  Standard Controls Assay Ready Controls Encapsulated Controls
Biosafety Level 1 Level 1 Level 1
Storage Temperature: -70°C to -90°C Temperature: -20°C Ambient     
(4°C - 40°C)
Specification Range Approximately 1x10^6 copies/μL Approximately 2x10^6 copies/tube Approximately 50,000 copies/tube
Physical State Frozen liquid Dried pellet Encapsulated dried pellet
Shelf Life 2 years 2 years 5 years
Shipping Dry Ice Ambient Ambient 
Sensitive Detection
Fully synthetic RNA
Includes fully synthetic RNA generated from Twist gene fragments
Powerful alternative to live viruses
Sequencing
Full viral genome coverage
>99.9% viral genome coverage
NGS sequence verified
Enables versatility in assay design
Versatile controls
Versatile controls
Positive controls perform for both qPCR and NGS assays
Content is well-aligned with Twist target enrichment panels
New Control Variants Cover the Recent Strains That Impact the World

Now available: Variants of Interest and Variants of Concern, see table on SARS-CoV-2 Controls tab

•    Control 14 Alpha (B.1.1.7 lineage) 
•    Control 15 Alpha (B.1.1.7 lineage) 
•    Control 16 Beta (B.1.351 lineage) 
•    Control 17 Gamma (P.1 lineage) 
•    Control 18 Kappa (B.1.617.1 lineage) 
•    Control 19 Iota (B.1.526 lineage)
•    Control 21 Epsilon (B1.1.429 lineage)
•    Control 23 Delta (B.1.617.2 lineage) 
•    Control 24 (B.1.617.3 lineage) 
•    Control 28 Delta (AY.1 lineage) 
•    Control 29 Delta (AY.2 lineage)
•    Control 48 Omicron (B.1.1.529, BA.1 lineage)
•    Control 50 Omicron (B.1.1.529, BA.2 lineage) 
•    Control 51 Omicron (B.1.1.529, BA.2 lineage) 

TARGETED TOOLS FOR SARS-CoV-2 RESEARCH

Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.

Watch the Webinar

Trusted, accurate and safe controls for
a wide-range of applications

The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing. 

 

Reproducible, Quality Results

Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays. 

 

A Safe and Effective Alternative to “Live Virus”

Controls based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.

 

Flexible formats for your applications

Choose from Standard, Assay Ready, or Encapsuled Control formulations. The standard format provides the controls at high concentration in a frozen liquid formulation. The Assay Ready Controls are provided as a dried pellet that increases stability. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s The Encapsulated Control products leverage a metal capsule sealed around a desiccated pellet to enhance stability of the RNA controls.
 
Both the Assay Ready and the Encapsulated formulations are single use controls. The Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers. The enhanced stability of the Encapsulated controls enables extended shelf life and both ship and store at room temperature. Ambient shipping reduces cost and increases accessibility for researchers across the globe. 

 

  Standard Controls Assay Ready Controls Encapsulated Controls
Biosafety Level 1 Level 1 Level 1
Storage Temperature: -70°C to -90°C Temperature: -20°C Ambient     
(4°C - 40°C)
Specification Range Approximately 1x10^6 copies/μL Approximately 2x10^6 copies/tube Approximately 50,000 copies/tube
Physical State Frozen liquid Dried pellet Encapsulated dried pellet
Shelf Life 2 years 2 years 5 years
Shipping Dry Ice Ambient Ambient 
Sensitive Detection
Fully synthetic RNA
Includes fully synthetic RNA generated from Twist gene fragments
Powerful alternative to live viruses
Sequencing
Full viral genome coverage
>99.9% viral genome coverage
NGS sequence verified
Enables versatility in assay design
Versatile controls
Versatile controls
Positive controls perform for both qPCR and NGS assays
Content is well-aligned with Twist target enrichment panels
New Control Variants Cover the Recent Strains That Impact the World

Now available: Variants of Interest and Variants of Concern, see table on SARS-CoV-2 Controls tab

•    Control 14 Alpha (B.1.1.7 lineage) 
•    Control 15 Alpha (B.1.1.7 lineage) 
•    Control 16 Beta (B.1.351 lineage) 
•    Control 17 Gamma (P.1 lineage) 
•    Control 18 Kappa (B.1.617.1 lineage) 
•    Control 19 Iota (B.1.526 lineage)
•    Control 21 Epsilon (B1.1.429 lineage)
•    Control 23 Delta (B.1.617.2 lineage) 
•    Control 24 (B.1.617.3 lineage) 
•    Control 28 Delta (AY.1 lineage) 
•    Control 29 Delta (AY.2 lineage)
•    Control 48 Omicron (B.1.1.529, BA.1 lineage)
•    Control 50 Omicron (B.1.1.529, BA.2 lineage) 
•    Control 51 Omicron (B.1.1.529, BA.2 lineage) 

TARGETED TOOLS FOR SARS-CoV-2 RESEARCH

Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.

Watch the Webinar

SARS-CoV-2 Controls
The use of positive controls in quality control

Twist has created synthetic RNA controls for several viral genomes. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome. The majority of the controls are provided in the standard format which consist of 100 μL at a concentration of approximately one million copies per microliter. Twist also offers certain controls in Assay Ready and Encapsulated Formats that are supplied as a desiccated pellet. The Assay Ready Controls are supplied at approximately two million copies per tube. The Encapsulated Controls are supplied at approximately 50,000 copies per tube. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control was developed in partnership with the US CDC and integrated into the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which tests for influenza A, B and SARS-CoV-2 simultaneously. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control is now available for customers outside the CDC. 


Twist Bioscience Encapsulated Control products offer a unique storage and delivery method for Twist’s established Synthetic SARS-CoV-2 RNA controls. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s Encapsulated Control products leverage a novel method to seal a metal capsule around a desiccated pellet to enhance stability of the RNA controls. Due to the enhanced stability, they can be shipped and stored at room temperature, reducing cost and improving accessibility globally. The Encapsulated Controls are sold in a rack of 16 or 96 tubes, are single use, and have a 5-year shelf life from the date of manufacture.

 

 

Available SARS-CoV-2 Controls

Best practices for using Assay Ready and Encapsulated Controls
 

For the Storage and use of Assay Ready or Encapsulated SARS-CoV-2 Controls please review the Following documents:

NGS SARS-CoV-2 Synthetic RNA Controls Genome

 

 

PART # CONTROL GenBank/GISAID ID GISAID NAME
102019 Control 1 MT007544.1 Australia/VIC01/2020
102024 Control 2 MN908947.3 Wuhan-Hu-1
103925 Assay Ready Control 2 MN908947.3 Wuhan-Hu-1
104422 Encapsulated Control 2 (16) MN908947.3 Wuhan-Hu-1
104426 Encapsulated Control 2 (96) MN908947.3 Wuhan-Hu-1
102860 Control 3 LC528232.1 Japan/Hu_DP_Kng_19-020/2020
102862 Control 4 MT106054.1 USA/TX1/2020
102917 Control 5 MT188340 USA/MN2-MDH2/2020
102918 Control 6 MT118835 USA/CA9/2020
103087 Control 7 EPI_ISL_418227 France/HF2393/2020
103511 Control 8 MT066176 Taiwan/NTU02/2020
103512 Control 9 MT152824 USA/WA2/2020
103513 Control 10 EPI_ISL_414648 USA/CA-PC101P/2020
103514 Control 11 EPI_ISL_417739 Iceland/5/2020
103515 Control 12 EPI_ISL_420244 England/SHEF-C05B2/2020
103533 Control 13 EPI_ISL_421184 Belgium/ULG-10004/2020
103907 Control 14
Alpha
EPI_ISL_710528 England/205041766/2020
103926 Assay Ready Control 14
Alpha (B.1.1.7)
EPI_ISL_710528 England/205041766/2020
103909 Control 15
Alpha
EPI_ISL_601443 England/MILK-9E05B3/2020
103927

Assay Ready Control 15
Alpha (B.1.1.7)

EPI_ISL_601443 England/MILK-9E05B3/2020
104043 Control 16
Beta (B.1.351)
EPI_ISL_678597 South Africa/KRISP-EC-K005299/2020
104044 Control 17
Gamma (P.1)
EPI_ISL_792683 Japan (Brazil) /IC-0564/2021
104338

Control 18
Kappa (B.1.617.1)

EPI_ISL_1662307 India/CT-ILSGS00361/2021
104529

Control 19
Iota (B.1.526)

EPI_ISL_1300881 USA/NY-MSHSPSP-PV24650/2020
104530 Control 20 (B.1.427) EPI_ISL_730092 USA/CA-ALSR-4704/2020
104531

Control 21
Epsilon (B.1.429)

EPI_ISL_672365 USA/CA-CZB-12943/2020
104532 Control 22 (B1.1.519) EPI_ISL_933685 Mexico/CMX-InDRE_208/2021

104533

Control 23
Delta (B.1.617.2)
EPI_ISL_1544014 India/MH-NCCS-P1162000182735/2021
104534 Control 24
(B.1.617.3)
EPI_ISL_1939891 India/MH-SEQ-221_S66_R1_001/2021
104538

Control 28  
Delta (AY.1)

EPI_ISL_2695467 Portugal/PT9543/2021
104423 Encapsulated Control 28 (16) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104427 Encapsulated Control 28 (96) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104539

Control 29  
Delta (AY.2)

EPI_ISL_2693246 USA/WA-CDC-UW21061750277/2021
105204 Control 48 (B.1.1.529/BA.1) EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104424 Encapsulated Control 48 (16)
(B.1.1.529/BA.1)
EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104428 Encapsulated Control 48 (96)
(B.1.1.529/BA.1)
EPI_ISL_6841980

Hong Kong/HKU-211129-001/2021

105345 Control 50 (B.1.1.529+BA.2) EPI_ISL_7190366 Australia/QLD2568/2021
105346 Control 51 (B.1.1.529+BA.2) EPI_ISL_7718520 England/MILK-2DF642C/2021

 

 

 

The use of positive controls in quality control

Twist has created synthetic RNA controls for several viral genomes. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome. The majority of the controls are provided in the standard format which consist of 100 μL at a concentration of approximately one million copies per microliter. Twist also offers certain controls in Assay Ready and Encapsulated Formats that are supplied as a desiccated pellet. The Assay Ready Controls are supplied at approximately two million copies per tube. The Encapsulated Controls are supplied at approximately 50,000 copies per tube. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control was developed in partnership with the US CDC and integrated into the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which tests for influenza A, B and SARS-CoV-2 simultaneously. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control is now available for customers outside the CDC. 


Twist Bioscience Encapsulated Control products offer a unique storage and delivery method for Twist’s established Synthetic SARS-CoV-2 RNA controls. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s Encapsulated Control products leverage a novel method to seal a metal capsule around a desiccated pellet to enhance stability of the RNA controls. Due to the enhanced stability, they can be shipped and stored at room temperature, reducing cost and improving accessibility globally. The Encapsulated Controls are sold in a rack of 16 or 96 tubes, are single use, and have a 5-year shelf life from the date of manufacture.

 

 

Available SARS-CoV-2 Controls

Best practices for using Assay Ready and Encapsulated Controls
 

For the Storage and use of Assay Ready or Encapsulated SARS-CoV-2 Controls please review the Following documents:

NGS SARS-CoV-2 Synthetic RNA Controls Genome

 

 

PART # CONTROL GenBank/GISAID ID GISAID NAME
102019 Control 1 MT007544.1 Australia/VIC01/2020
102024 Control 2 MN908947.3 Wuhan-Hu-1
103925 Assay Ready Control 2 MN908947.3 Wuhan-Hu-1
104422 Encapsulated Control 2 (16) MN908947.3 Wuhan-Hu-1
104426 Encapsulated Control 2 (96) MN908947.3 Wuhan-Hu-1
102860 Control 3 LC528232.1 Japan/Hu_DP_Kng_19-020/2020
102862 Control 4 MT106054.1 USA/TX1/2020
102917 Control 5 MT188340 USA/MN2-MDH2/2020
102918 Control 6 MT118835 USA/CA9/2020
103087 Control 7 EPI_ISL_418227 France/HF2393/2020
103511 Control 8 MT066176 Taiwan/NTU02/2020
103512 Control 9 MT152824 USA/WA2/2020
103513 Control 10 EPI_ISL_414648 USA/CA-PC101P/2020
103514 Control 11 EPI_ISL_417739 Iceland/5/2020
103515 Control 12 EPI_ISL_420244 England/SHEF-C05B2/2020
103533 Control 13 EPI_ISL_421184 Belgium/ULG-10004/2020
103907 Control 14
Alpha
EPI_ISL_710528 England/205041766/2020
103926 Assay Ready Control 14
Alpha (B.1.1.7)
EPI_ISL_710528 England/205041766/2020
103909 Control 15
Alpha
EPI_ISL_601443 England/MILK-9E05B3/2020
103927

Assay Ready Control 15
Alpha (B.1.1.7)

EPI_ISL_601443 England/MILK-9E05B3/2020
104043 Control 16
Beta (B.1.351)
EPI_ISL_678597 South Africa/KRISP-EC-K005299/2020
104044 Control 17
Gamma (P.1)
EPI_ISL_792683 Japan (Brazil) /IC-0564/2021
104338

Control 18
Kappa (B.1.617.1)

EPI_ISL_1662307 India/CT-ILSGS00361/2021
104529

Control 19
Iota (B.1.526)

EPI_ISL_1300881 USA/NY-MSHSPSP-PV24650/2020
104530 Control 20 (B.1.427) EPI_ISL_730092 USA/CA-ALSR-4704/2020
104531

Control 21
Epsilon (B.1.429)

EPI_ISL_672365 USA/CA-CZB-12943/2020
104532 Control 22 (B1.1.519) EPI_ISL_933685 Mexico/CMX-InDRE_208/2021

104533

Control 23
Delta (B.1.617.2)
EPI_ISL_1544014 India/MH-NCCS-P1162000182735/2021
104534 Control 24
(B.1.617.3)
EPI_ISL_1939891 India/MH-SEQ-221_S66_R1_001/2021
104538

Control 28  
Delta (AY.1)

EPI_ISL_2695467 Portugal/PT9543/2021
104423 Encapsulated Control 28 (16) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104427 Encapsulated Control 28 (96) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104539

Control 29  
Delta (AY.2)

EPI_ISL_2693246 USA/WA-CDC-UW21061750277/2021
105204 Control 48 (B.1.1.529/BA.1) EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104424 Encapsulated Control 48 (16)
(B.1.1.529/BA.1)
EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104428 Encapsulated Control 48 (96)
(B.1.1.529/BA.1)
EPI_ISL_6841980

Hong Kong/HKU-211129-001/2021

105345 Control 50 (B.1.1.529+BA.2) EPI_ISL_7190366 Australia/QLD2568/2021
105346 Control 51 (B.1.1.529+BA.2) EPI_ISL_7718520 England/MILK-2DF642C/2021

 

 

 

Respiratory Virus Controls
Available Respiratory Virus Controls

The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design. 

The figure shows a taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.
 

NGS Respiratory Viral Controls Taxonomic Tree
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.

Contact your local sales representative for more details or Buy Now.

NGS Respiratory Viral Controls Part Numbers
Available Respiratory Virus Controls

The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design. 

The figure shows a taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.
 

NGS Respiratory Viral Controls Taxonomic Tree
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.

Contact your local sales representative for more details or Buy Now.

NGS Respiratory Viral Controls Part Numbers
RESOURCES