Pretty fast, but still not Express Genes fast.

Pretty fast, but still not Express Genes fast.

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Introducing Twist Express Genes

Clonal Genes made to your specifications and shipped to you in as few as 5 days.*


Fast track your research with Twist Express Genes. This new service provides you with NGS-verified, sequence perfect Clonal Genes, synthesized between 0.3-5 kb in length and cloned into the vector of your choice, all within 5-7 business days.* With fast production time and quality like this, you can expect speed and precision for all applications including antibody engineering, drug discovery research, mRNA synthesis, and many more.

Simple as 1-2-3!

Start Your Genes Design

Upload your gene sequences.

Select Express Genes

Upload your sequences, select your Express Genes customizations, and submit your order.

Sit Back and Relax

Your perfect NGS-verified Clonal Genes will be shipped in as few as 5 days.*

Ready to Accelerate and Transform Your Research with Express Genes?

FAQ

Express Genes are Clonal Genes produced faster using Twist’s Express service. The Express service reduces production time from the standard 10-15 business days down to 5-7 business days without compromising quality at any stage.*

50 ng - 2 µg & 2 µg - 10 µg DNA Scale:

Standard: 10-15 business days

Express: 5-7 business days

10 µg - 100 µg & 100 µg - 1mg DNA Scale:

Standard: 13-20 business days

Express: 8-12 business days

*Terms and Conditions: Advertised turnaround time for Express Clonal Genes is 5-7 business days and is based on extensive production experience. Your production time may vary from sequence-to-sequence. International orders may require additional shipping time. Express Genes fee is based on the capacity of the chip at time of order submission and will fluctuate.

Ordering Clonal Genes with our Express service ensures that an order receives top priority at every production stage. With additional resources dedicated to our new tier of service, this prioritized approach allows us to expedite the production of Clonal Genes without compromising quality.

No, the 5-7 business day turnaround time is the amount of time it takes to manufacture Express Genes.* 

All Clonal Genes (Express & Standard) are shipped using overnight or priority service.

Typical shipping times by region are as follows: 

  • North America: 1-2 business days
  • Europe: 2-3 business days
  • Asia: 2-4 business days
  • South America: 3-6 business days

Express Genes ordered with glycerol stocks and Express Genes at the 10 μg - 100 μg and 100 μg - 1 mg DNA Scales (i.e. resuspended DNA) are shipped on dry ice, which may add one business day to the overall turnaround time.

General considerations that may delay shipping and delivery include:

  • Issues with payment methods
  • Country-specific import requirements
  • Biosafety sequence screening

*Terms and Conditions: Advertised turnaround time for Express Clonal Genes is 5-7 business days and is based on extensive production experience. Your production time may vary from sequence-to-sequence. International orders may require additional shipping time. Express Genes fee is based on the capacity of the chip at time of order submission and will fluctuate.

No, new custom vectors will need to be onboarded prior to ordering Express Genes. New Custom Vector Onboarding will take approximately 2-3 weeks. For more information on Custom Vector Onboarding, please see the link to our FAQs for additional information.

Express genes are available in the following DNA prep sizes: 50 ng - 2 μg, 2 μg - 10 μg, 10 μg - 100 μg and 100 μg - 1 mg. Turnaround time increases with prep size and is as follows: 5-7 BD for 50 ng - 2 μg and 2 μg - 10 μg, and 8-12 BD for 10 μg - 100 μg and 100 μg - 1 mg. The same shipment format options are available for Standard and Express genes.

Clonal Gene sequences scored as “Standard” complexity are eligible for the Express service. The Twist Bioscience eCommerce platform will score all sequences once they are uploaded and flag which ones are eligible for the Express service.

See here for additional information on sequence scoring parameters. 

All Clonal Gene sequences between 300bp to 5000bp that are scored as “Standard” complexity by our screener are eligible for the Express service.

Our scoring system adopts a machine learning model which analyzes and combines multiple sequence parameters ( i.e. overall GC percent, maximum homopolymer length, maximum repeat length, sequence length, repeat density, etc.). This model was trained using our historical manufacturing data, and it provides a more precise estimation of production success for genes and antibody products.

Complexity issues are driven mainly by repetitive structures and extreme GC content. Guidelines for sequence design are available online and below:

  • Avoid repeats of ≥ 20bp or Tm ≥ 60C 
  • Global GC content must be between 25% and 65%
  • Avoid extreme differences in GC content within a gene (i.e. the difference in GC content between the highest and lowest 50 bp stretch should be no greater than 52%)
  • Minimize homopolymers
  • Minimize the number/length of small repeats scattered throughout the sequence
  • For HIS tags, use a combination of CAC and CAT codons i.e. CACCAT

The likelihood of sequence acceptance improves by limiting repeats and reducing GC content. For example: a gene may score as “Not Accepted” with 66% overall GC content, but when reduced to 65% or less, may be accepted as a “Complex” sequence. Further lowering GC content to ~60% or less may improve the complexity score to “Standard”, at which point the sequence would be eligible for the Express service. The same holds true for eliminating repeats, reducing homopolymer stretches, and evening out extreme variations in GC windows. The more such structures can be avoided or reduced in a gene, the more likely it is to qualify for the Express service.

For additional information on our scoring algorithm and sequence design, please visit our FAQs.

Complex sequences often require more production time to synthesize and clone, and therefore, are only available at the standard production speed.

The Express service is an upgraded service for Clonal Genes that provides a faster turnaround time than our Standard service. See below for a detailed breakdown on the differences between the Standard and Express services:

Yes, a fee is added to each gene with the Express service applied. The fee will not be invoiced for any Express Gene that does not ship within the advertised turnaround time.

Express Genes service employs dynamic pricing to ensure availability at all times and reliable on-time delivery, especially during high-demand periods. Whenever there's a change in pricing, it will be reflected on our eCommerce platform.

We continue to offer Clonal Genes produced with our Standard speed of service, which has a 10-15 business day turnaround time.

Contracted pricing will be reflected in the base price of each gene, and the Express service fee is added as a separate line item relative to that contracted base price.

Twist is committed to providing the best experience possible. In the event Express Genes are delivered late, the Express service fee will be discounted 100% and you will be invoiced at the Standard Clonal Gene price. Terms and conditions for the policy are here.

Additionally, we stand behind Express Genes with our Twist Satisfaction Policy. In the event that you are not satisfied with your Express Genes, please notify us within thirty (30) days of order placement.

Only “Standard” complexity sequences are eligible for the Express service. If a gene sequence is grayed out under the Express Genes section, that means it is a “Complex” or “Not accepted” sequence and will be marked as such in the eCommerce platform.

Yes, the maximum limit when configuring a project on eCommerce is 1152 Express Genes. If you plan on placing an order with more than 1152 Express Genes, please contact Customer Support for help submitting your order.

This indicates that we have reached maximum capacity for the current Express Genes run. Express Genes are run multiple times a day, please check back soon.

*Terms and conditions: Customizations for Express Genes include selecting from Twist Catalog Vectors or onboarding your own custom vector for cloning, sequence lengths ranging from 0.3 to 5.0 kb, and DNA prep scales of 50 ng - 2 μg, 2 μg - 10 μg, 10 μg - 100 μg, 100 μg - 1 mg. Turnaround time for Express Genes starts at 5-7 business days and increases to 8-12 business days for 10 μg - 100 μg and 100 μg - 1 mg DNA prep scales. Onboarding your own custom vector will incur 1-2 weeks additional turnaround time.

For Research Use Only. Not for Diagnostic Procedures.