In Silico Antibody Humanization

Cut Your Humanization Time in Half

OVERVIEW
BENEFITS
DATA
TECHNICAL RESOURCES

Library-Free, Machine Learning-Assisted Antibody Humanization

Antibody humanization can involve laborious library construction and monotonous cycles of protein engineering to design a fully humanized, antibody candidate that is developable and retains the same affinity as the parental antibody.

Twist Biopharma Solutions has developed computational pipelines for the automated generation of humanized variants using a combination of:

Structural modeling

Machine Learning

Deep Learning

CDR grafting

Using this in silico humanization platform to rapidly generate humanized antibody candidates from murine, VHH, or chimeric antibody hits, bypasses the need to build and mine an antibody library, cutting down the time to deliver a humanized antibody by more than half.

How it works

Maintain Affinity, Safety, and Efficacy Profiles

In Silico-Powered, Derisked Antibody Humanization

We’ve developed and validated a powerful machine learning algorithm – one trained on millions of human antibody sequences – to enable Twist to go beyond guesswork and instead design humanized antibody sequences that are highly likely to retain both affinity, functionality, and developability.

A Workflow Shaped by Deep Expertise

Our team of antibody experts has honed our humanization process for a variety of antibody hits. Using our in silico platform, we input heavy and light chains (or heavy chain only) and utilize a combination of structural modeling, ML approaches, and CDR grafting to generate humanized variants.

Complementary Capabilities to Take You Closer to the Clinic

Following humanization, expression, characterization of binding affinity or further optimization of binding affinity, selectivity, and PK may be needed. Twist can quickly advance your newly humanized candidates closer to the clinic with high-throughput clonal gene synthesis, rapid antibody expression, affinity testing, and Twist Antibody Optimization.

Fully Humanized Affinity Assessment

Using Twist’s rapid humanization pipeline all three murine antibodies were successfully humanized. For a few candidates, the affinity of the top humanized variant was even modestly improved compared to its chimeric control.