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OVERVIEW

Comprehensive Cancer Variant Reference Standard for Liquid Biopsy Research

 

NGS-based liquid biopsy is a rapidly evolving application that requires accurate and precise reference standards. The Twist Pan-cancer Reference Standard is a high-quality, standardized control for use in the research and development of NGS-based liquid biopsy assays. This reference standard is ideal for establishing the analytical limit of detection (LoD) for specific cancer variants and as a control to track the quality of an NGS assay workflow and assess the fidelity of the assay process during research and development of such assays.
 

Traditional reference standards are limited in the number and variation of variants and typically use cell line-derived DNA which can carry unwanted sequence variations and variable fragment length. The Twist cfDNA Pan-cancer Reference Standard incorporates a large and diverse set of variants for research use. This reference standard utilizes accurate DNA synthesis of variants with precise control over the specific variant allele frequencies (VAF) to produce a reference standard which closely mimics the size distribution and fragmentation profile of cell-free and circulating tumor DNA enabling higher performance assay development.
 

This reference standard consists of synthetically designed variant sequences that mimic circulating tumor DNA (ctDNA), combined with background DNA that is derived from, and closely mimics, human-derived cell-free DNA (cfDNA). The ctDNA sequences are designed as a tiled pool of ~167 bp sequences that closely mimic natural ctDNA and cover 458 individual mutations with 132 clinically actionable variants across 84 genes associated with cancer.

 

Comprehensive Detection of Cancer Variants
Comprehensive Detection of Cancer Variants
458 unique naturally occurring cancer variants
132 clinically relevant variants
Covers 84 different genes involved in cancer
Better Design of Background DNA
Better Design of Background DNA
Background DNA derived from human cfDNA samples
DNA size profile and post-sequencing profile mimic native cfDNA
Designed for Precision and Flexibility
Designed for Precision and Flexibility
Seven individual VAF percentages to choose from
Digital Droplet PCR verification of VAF percentages
Convenient test set of all VAF percentages available

*For research use only. Not for use in any diagnostic or clinical procedures.

CONTACT US
 

Comprehensive Cancer Variant Reference Standard for Liquid Biopsy Research

 

NGS-based liquid biopsy is a rapidly evolving application that requires accurate and precise reference standards. The Twist Pan-cancer Reference Standard is a high-quality, standardized control for use in the research and development of NGS-based liquid biopsy assays. This reference standard is ideal for establishing the analytical limit of detection (LoD) for specific cancer variants and as a control to track the quality of an NGS assay workflow and assess the fidelity of the assay process during research and development of such assays.
 

Traditional reference standards are limited in the number and variation of variants and typically use cell line-derived DNA which can carry unwanted sequence variations and variable fragment length. The Twist cfDNA Pan-cancer Reference Standard incorporates a large and diverse set of variants for research use. This reference standard utilizes accurate DNA synthesis of variants with precise control over the specific variant allele frequencies (VAF) to produce a reference standard which closely mimics the size distribution and fragmentation profile of cell-free and circulating tumor DNA enabling higher performance assay development.
 

This reference standard consists of synthetically designed variant sequences that mimic circulating tumor DNA (ctDNA), combined with background DNA that is derived from, and closely mimics, human-derived cell-free DNA (cfDNA). The ctDNA sequences are designed as a tiled pool of ~167 bp sequences that closely mimic natural ctDNA and cover 458 individual mutations with 132 clinically actionable variants across 84 genes associated with cancer.

 

Comprehensive Detection of Cancer Variants
Comprehensive Detection of Cancer Variants
458 unique naturally occurring cancer variants
132 clinically relevant variants
Covers 84 different genes involved in cancer
Better Design of Background DNA
Better Design of Background DNA
Background DNA derived from human cfDNA samples
DNA size profile and post-sequencing profile mimic native cfDNA
Designed for Precision and Flexibility
Designed for Precision and Flexibility
Seven individual VAF percentages to choose from
Digital Droplet PCR verification of VAF percentages
Convenient test set of all VAF percentages available

*For research use only. Not for use in any diagnostic or clinical procedures.

CONTACT US
 
DATA
Comparison of Twist’s Reference standard and competitors

DNA analysis of the Twist cfDNA Pan-Cancer Reference Standard and competitors reference standards show that Twist more closely mimics native cell-free DNA providing a more true to life test result.

fluorescent units
Post Sequencing Profile Comparison

Post sequencing library size distribution comparison between Twist and competitors show how similar Twist mimics native cfDNA samples including the jagged leading peaks.

library insert size
Comparison of Twist’s Reference standard and competitors

DNA analysis of the Twist cfDNA Pan-Cancer Reference Standard and competitors reference standards show that Twist more closely mimics native cell-free DNA providing a more true to life test result.

fluorescent units
Post Sequencing Profile Comparison

Post sequencing library size distribution comparison between Twist and competitors show how similar Twist mimics native cfDNA samples including the jagged leading peaks.

library insert size
ORDERING INFORMATION
PRODUCT SKUS

104549

*Twist cfDNA Pan-cancer reference standard set, 300 ng per tube 

104563

Twist cfDNA Pan-cancer reference standard VAF 0% (WT), 3ug

104564

Twist cfDNA Pan-cancer reference standard VAF 0.1 %, 3ug

104565

Twist cfDNA Pan-cancer reference standard VAF 0.25 %, 3ug

104566

Twist cfDNA Pan-cancer reference standard VAF 0.5 %, 3ug

104567

Twist cfDNA Pan-cancer reference standard VAF 1 %, 3ug

104568

Twist cfDNA Pan-cancer reference standard VAF 2 %, 3ug

104569

Twist cfDNA Pan-cancer reference standard VAF 5 %, 3ug

104176

Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 16 Samples

104177

Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 96 Samples

*Kit includes 7 VAFs individually: 0% (WT), 0.1%, 0.25%, 0.5%, 1%, 2%, 5% at 300ng each tube

*For research use only. Not for use in any diagnostic or clinical procedures.

PRODUCT SKUS

104549

*Twist cfDNA Pan-cancer reference standard set, 300 ng per tube 

104563

Twist cfDNA Pan-cancer reference standard VAF 0% (WT), 3ug

104564

Twist cfDNA Pan-cancer reference standard VAF 0.1 %, 3ug

104565

Twist cfDNA Pan-cancer reference standard VAF 0.25 %, 3ug

104566

Twist cfDNA Pan-cancer reference standard VAF 0.5 %, 3ug

104567

Twist cfDNA Pan-cancer reference standard VAF 1 %, 3ug

104568

Twist cfDNA Pan-cancer reference standard VAF 2 %, 3ug

104569

Twist cfDNA Pan-cancer reference standard VAF 5 %, 3ug

104176

Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 16 Samples

104177

Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 96 Samples

*Kit includes 7 VAFs individually: 0% (WT), 0.1%, 0.25%, 0.5%, 1%, 2%, 5% at 300ng each tube

*For research use only. Not for use in any diagnostic or clinical procedures.

RESOURCES