Don’t Settle for Less
Don’t Settle for Less
Don’t Settle for Less
OVERVIEW DATA CUSTOMIZATION ORDERING & SPECS RESOURCES
OVERVIEW

Exome sequencing has become a widely used practice in clinics and diagnostics. The Twist Comprehensive Exome Panel offers coverage of greater than 99% of protein coding genes. The panel’s superior performance provides the optimal exome sequencing solution, while focusing on the most accurate curated subset—CCDS database. Created to include expanded content of RefSeq and GENCODE databases. 

 

 

Exceptional performance
More Complete Coverage
Covers 36.8 Mb of human protein coding regions
Based on recent database releases
Retain Flexibility
Retain Flexibility
Easily spike-in content into comprehensive exome panel
Effective across multiplex target enrichment workflows
Design Efficiency
Design Efficiency
The panel targets 36.8 Mb with a design size of only 41.2 Mb
Smaller design size reduces sequencing costs
EXOME DEFINITION

99.3% of targeted regions covered at 20x with 5.3 Gb

Targets 36.8 Mb with a design size of only 41.2 Mb

Covers greater than 99% coverage of protein coding genes

Exome sequencing has become a widely used practice in clinics and diagnostics. The Twist Comprehensive Exome Panel offers coverage of greater than 99% of protein coding genes. The panel’s superior performance provides the optimal exome sequencing solution, while focusing on the most accurate curated subset—CCDS database. Created to include expanded content of RefSeq and GENCODE databases. 

 

 

Exceptional performance
More Complete Coverage
Covers 36.8 Mb of human protein coding regions
Based on recent database releases
Retain Flexibility
Retain Flexibility
Easily spike-in content into comprehensive exome panel
Effective across multiplex target enrichment workflows
Design Efficiency
Design Efficiency
The panel targets 36.8 Mb with a design size of only 41.2 Mb
Smaller design size reduces sequencing costs
EXOME DEFINITION

99.3% of targeted regions covered at 20x with 5.3 Gb

Targets 36.8 Mb with a design size of only 41.2 Mb

Covers greater than 99% coverage of protein coding genes

DATA
Increase Confidence in Your Variant Detection Experiments

Gene definitions are constantly being updated by dedicated consortia such as GENCODE, based on new information from large scale experiments. Thus, panels based on definitions from even several years ago may be missing a considerable portion of genes, and conversely may include some outdated sequences.

The Comprehensive Exome Panel targets 36.8 Mb of human protein coding genes, covering >99% of RefSeq, CCDS, and GENCODE databases. This is best in class coverage of the most up-to-date content, when compared to competitive products (see table below).
 

NGS-Fixed-Panel-Human-Comprehensive-Exome-Databases-Venn
Best-in-Class Coverage Compared to the Competition

Coverage comparison of different databases among several commercially available exomes. This is calculated by overlap between target content versus protein coding exons in databases annotated on primary human genome assembly as of May 2019 UCSC genome browser.

+ Human Genome Version 19
+ Human Genome Version 38

NGS-Fixed-Panel-Human-Comprehensive-Exome-Databases-Table
Maximum Sequence Efficiency

Target coverage (>94%) is far superior using either the Exome only and the Comprehensive Exome Panel compared to other market equivalent kits.

NGS-Fixed-Panel-Human-Comprehensive-Exome-TargetCoverage-Graph
Best Coverage Uniformity

Fold-80 (1.34–1.36) is far superior using either the Exome only and the Comprehensive Exome Panel compared to other market equivalent kits.

NGS-Fixed-Panel-Human-Comprehensive-Exome-Fold80-Graph
Increase Confidence in Your Variant Detection Experiments

Gene definitions are constantly being updated by dedicated consortia such as GENCODE, based on new information from large scale experiments. Thus, panels based on definitions from even several years ago may be missing a considerable portion of genes, and conversely may include some outdated sequences.

The Comprehensive Exome Panel targets 36.8 Mb of human protein coding genes, covering >99% of RefSeq, CCDS, and GENCODE databases. This is best in class coverage of the most up-to-date content, when compared to competitive products (see table below).
 

NGS-Fixed-Panel-Human-Comprehensive-Exome-Databases-Venn
Best-in-Class Coverage Compared to the Competition

Coverage comparison of different databases among several commercially available exomes. This is calculated by overlap between target content versus protein coding exons in databases annotated on primary human genome assembly as of May 2019 UCSC genome browser.

+ Human Genome Version 19
+ Human Genome Version 38

NGS-Fixed-Panel-Human-Comprehensive-Exome-Databases-Table
Maximum Sequence Efficiency

Target coverage (>94%) is far superior using either the Exome only and the Comprehensive Exome Panel compared to other market equivalent kits.

NGS-Fixed-Panel-Human-Comprehensive-Exome-TargetCoverage-Graph
Best Coverage Uniformity

Fold-80 (1.34–1.36) is far superior using either the Exome only and the Comprehensive Exome Panel compared to other market equivalent kits.

NGS-Fixed-Panel-Human-Comprehensive-Exome-Fold80-Graph
CUSTOMIZATION
Easy to Add Content Using Twist Target Enrichment

The Twist Comprehensive Exome Panel can be combined with other targets you require, providing flexibility in content while retaining high uniformity.

Customization of Core Exome Kit Content and Components
Easy to Add Content Using Twist Target Enrichment

The Twist Comprehensive Exome Panel can be combined with other targets you require, providing flexibility in content while retaining high uniformity.

Customization of Core Exome Kit Content and Components
ORDERING & SPECS
PRODUCT SKUS
102031 Twist Comprehensive Exome, 2 Reactions, Kit Panel for 2 enrichment reactions targeting 36.8 Mb of human protein coding regions 
102032 Twist Comprehensive Exome, 12 Reactions, Kit Panel for 12 enrichment reactions targeting 36.8 Mb of human protein coding regions 
102033 Twist Comprehensive Exome, 96 Reactions, Kit Panel for 96 enrichment reactions targeting 36.8 Mb of human protein coding regions
SPECIFICATIONS
Workflow Step Target Enrichment
Format dsDNA
Panel size 36.8 Mb
Content Human protein coding genes
Design Databases CCD, SRefSeq, GENCODE
Storage Store at -5 to -30°C

The Twist Synthetic SARS-CoV-2 RNA Controls and the Twist Respiratory Virus Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

PRODUCT SKUS
102031 Twist Comprehensive Exome, 2 Reactions, Kit Panel for 2 enrichment reactions targeting 36.8 Mb of human protein coding regions 
102032 Twist Comprehensive Exome, 12 Reactions, Kit Panel for 12 enrichment reactions targeting 36.8 Mb of human protein coding regions 
102033 Twist Comprehensive Exome, 96 Reactions, Kit Panel for 96 enrichment reactions targeting 36.8 Mb of human protein coding regions
SPECIFICATIONS
Workflow Step Target Enrichment
Format dsDNA
Panel size 36.8 Mb
Content Human protein coding genes
Design Databases CCD, SRefSeq, GENCODE
Storage Store at -5 to -30°C

The Twist Synthetic SARS-CoV-2 RNA Controls and the Twist Respiratory Virus Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

RESOURCES