Alliance Panels
Alliance Panels
Alliance Panels
OVERVIEW
OVERVIEW

Overview 

Introducing Twist Alliance Panels, a curated collection of high-quality target enrichment panels designed in partnership with leading scientific institutions. 

In combining Twist’s world-class oligonucleotide synthesis platform with our scientific partners’ deep area expertise, Twist Alliance panels provide ready-made panels to help accelerate your research. Why spend time designing panels when experts have already done the work for you?

Partner With Us

Are you interested in building a panel that is not already part of the Twist Alliance Panel portfolio? 

Partner with us to commercialize

 

Overview 

Introducing Twist Alliance Panels, a curated collection of high-quality target enrichment panels designed in partnership with leading scientific institutions. 

In combining Twist’s world-class oligonucleotide synthesis platform with our scientific partners’ deep area expertise, Twist Alliance panels provide ready-made panels to help accelerate your research. Why spend time designing panels when experts have already done the work for you?

Partner With Us

Are you interested in building a panel that is not already part of the Twist Alliance Panel portfolio? 

Partner with us to commercialize

 

Explore Our Panels

The Twist Alliance Panels are designed to give you a head start, bringing together expertise from multiple disciplines for superior target enrichment, for applications ranging from cancer diagnostics to carrier screening. If none of the panels look quite right for your needs, each panel can be customized with spike-in coverage where you need it. Are you interested in partnering with us to commercialize a new panel? Contact us.

Helping you skip the design, build, test, learn cycle.

Twist Alliance Diversity SNP Panel

A prevalent issue with historical genotyping tools is their bias toward a small number of ethnicities, leaving significant portions of humanity underrepresented in classic genotyping workflows. As an example, microarray platforms only offer fixed content updated occasionally, compounding this challenge.

Researchers can solve these inefficiencies and inequities by utilizing target capture-based NGS workflows with probe panels designed for diversity and flexibility.

As part of Twist’s growing Targeted GBS portfolio, the Twist Diversity SNP Panel gives researchers a new flexible ethnicity-neutral gold standard to use for GBS.

Scientists have the freedom to use the panel as a stand-alone genotyping panel or as a spike-in into the Twist Human Comprehensive Exome panel. Its design is also customizable—additional probes covering regions of interest can be synthesized on Twist's DNA synthesis platform.

The Twist Diversity SNP Panel content was curated in partnership with leaders at the Regeneron Genetics Center. Designed to integrate into existing fully automated exome processing workflows, this panel provides base calls and imputed variants.

Genotype imputations can be calculated using open source tools available. Please reach out to Customer Support to get a copy of our imputation guide to getting started with imputation analysis tools.
 

Note: The Diversity SNP panel content has not been fully validated through the complete Twist workflow. For recommendations on assay set-up, please contact Twist Customer Support.

PROBES

640k

SNPs COVERED

1.4M

SENSITIVITY

Imputation of most variants down to MAF of 0.1% or less

Ordering Information

104374

Twist Diversity SNP Panel, 2 Reactions

104375

Twist Diversity SNP Panel, 12 Reactions

104376

Twist Diversity SNP Panel, 96 Reactions

104393

Twist Diversity SNP Panel, 16 samples complete kit with Standard Hyb

104394

Twist Diversity SNP Panel, 96 samples complete kit with Standard Hy

Twist Alliance Pan-cancer Methylation Panel - 1.5 MB

Methylation sequencing is a sensitive tool for detecting and tracing the origin of cancer cells. But like any sequencing project, there is a tradeoff between breadth and depth of coverage. If you’re ready to focus on cancer-specific methylation patterns, this is the panel for you. Experts at AnchorDx partnered with Twist to create a custom target enrichment panel that focuses specifically on targets relevant to 31 different cancers. 

The Twist Alliance Pan-cancer Methylation Panel - 1.5 MB provides deep coverage of clinically focused targets that allow for the study of methylation patterns that may be relevant to early cancer detection and diagnosis from tumor and liquid biopsy samples. Designed in collaboration with experts at AnchorDx and constructed using Twist’s world-class oligonucleotide synthesis platform, this panel provides a high on-target rate and high uniformity, and has been validated with the Twist Methylation workflow using liquid biopsy samples of breast cancer, colorectal cancer, and non-small cell lung cancer.

 

Panel Design

  • 31 cancer types, 47 disease entities, based on TCGA database
  • 13,090 Probes 
  • 126k CpG sites involved
  • ~12k Differentially Methylated Regions (DMRs)
  • 9k of 12k (74%) overlapped with the CpG islands

Note: The Twist Alliance Pan-cancer Methylation Panel - 1.5 MB is not ISO-13485 certified

Ordering Information

104694

Twist Alliance Pan-cancer Methylation Panel - 1.5 MB, 2 Reactions

104695

Twist Alliance Pan-cancer Methylation Panel - 1.5 MB, 12 Reactions

104696

Twist Alliance Pan-cancer Methylation Panel - 1.5 MB, 96 Reactions
Twist Methylation Workflow

101976

NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing, 96 Samples*

103557

Twist Methylation Enhancer, 12 Reactions

100578

Twist Universal Blocker, 12 Reactions

100983

Twist Binding and Purification Beads Kit, 12 Reactions

101174

Twist Fast Hybridization and Wash Kit, 12 Reactions

103496

Twist Targeted Methylation Sequencing Workflow, 96x12 Reactions

*2 reaction and 12 reaction sizes are also available for enrichment

Twist Alliance Clinical Research Exome - 34.9 MB

The Twist Alliance Clinical Research Exome helps support the Broad Institute Genomics Platform and was designed using validated data from clinical patient samples. The panel leverages Twist’s flexible NGS platform to customize content easily and quickly, resulting in a comprehensive survey of the exome with supplemental enrichment of clinically relevant areas of the genome related to cancer as well as rare and inherited diseases. By leveraging the best-in-class uniformity of Twist NGS probes, the assay enables a per sample cost and throughput efficiency that Broad has already leveraged to process more than 250,000 samples to date, keeping it on the leading edge of exome sequencing.

The full design of this panel includes the Twist Core exome, the mitochondrial genome, and additional validated coding and non-exonic regions of interest such as the ACMG73 genes, supplemental coverage of regions from OMIM and COSMIC, and specific Broad-defined targets. 

Note: The Twist Alliance Clinical Research Exome - 34.9 MB content has not been fully validated through the complete Twist workflow. For recommendations on assay set-up, please contact Twist Customer Support. 

Note: The Twist Alliance Clinical Research Exome - 34.9 MB is not ISO-13485 certified.

 

Ordering Information

104032

Twist Alliance Clinical Research Exome - 34.9 MB, 2 Reactions Kit

104033

Twist Alliance Clinical Research Exome - 34.9 MB, 12 Reactions Kit

104034

Twist Alliance Clinical Research Exome - 34.9 MB, 96 Reactions Kit

Twist Alliance VCGS Exome - 40.1 MB

Where routine testing has failed to provide a diagnosis, physicians often turn to DNA sequencing panels for answers, particularly when a heritable disease is suspected. But without expert guidance, it can be hard to know which panel is the right panel.
Drawing on decades of experience in clinical genomics, Victorian Clinical Genetics Services (VCGS) has partnered with Twist to design a diagnostic panel—Twist Alliance VCGS Exome - 40.1 MB—that covers the whole exome, with dedicated coverage boosting over clinically relevant genes (Mendeliome). In designing this panel, special care was given to ensuring additional coverage of clinically relevant loci that fall outside the gene coding regions traditionally targeted by exomes (i.e. known pathogenic loci in non-coding regions).
The Twist Alliance VCGS Exome - 40.1 MB panel is a best-in-class, highly uniform panel that can help streamline the identification of heritable disease-linked alleles, be it through carrier screening or pre- and post-natal testing.

Note: The Twist Alliance VCGS Exome - 40.1 MB content has not been fully validated through the complete Twist workflow. For recommendations on assay set-up, please contact Twist Customer Support.

Note: The Twist Alliance VCGS Exome - 40.1 MB is not ISO-13485 certified.

Total probes

425.4K

Includes the Twist Comprehensive Exome of

394.1K probes

and content curated by VCGS of

31.3K probes

Ordering Information

104282

Twist Alliance VCGS Exome - 40.1 MB, 2 Reactions Kit

104283

Twist Alliance VCGS Exome - 40.1 MB, 12 Reactions Kit

104284

Twist Alliance VCGS Exome - 40.1 MB, 96 Reactions Kit

Twist Alliance Canine Exome - 40.5 Mb

For Research Use Only. Not for use in diagnostic procedures

Understanding the genetic variation between dog breeds is critical to understanding genetic regulation of traits as well as the basis and progression of disease in dogs. Consequently, comprehensive gene panels such as exomes are pivotal in improving veterinary diagnostics and the associated clinical medicine. These types of NGS panels will also accelerate research into developing a deeper understanding of canine cancers and potential therapeutics.

The Twist Alliance Canine Exome developed in collaboration with the Broad Institute’s Karlsson lab provides just the right tool for canine genomic research. In addition, canine genomic research has also been found to have benefits for human medical research due to genetic similarities found between human and canine tumors such as CNVs, differential gene expressions, and structural chromosome abnormalities1.

The Twist Alliance Canine exome panel was designed with the following objectives in mind

  1. Covers coding exons of canine genes
  2. Enable comparative genomic studies between canine and human genomes
  3. Includes regions of known importance in human cancers
  4. Allows for cost-effective deep sequencing

The panel contains more than 17k Genes/targets based on CanFam3.1, with additional coverage of genes/targets that are implicated in canine cancer. 
 

Genes

Associated
COSMIC
mutations

Genes Associated
COSMIC
mutations
Genes Associated
COSMIC
mutations
ABL1 19 HRAS 26 TET2 16
AKT1 6 MPL 0 FAS 3
ERBB2 19 SMAD4 31 STAT5B 4
GNAS 12 ATM 24 DNMT3A 6
MET 18 FBXW7 25 STAT3 6
RB1 18 IDH1 15 CREBBP 9
CCR4 3 NOTCH1 20 RHOA 1
MYC 5 SMARCB1 11 CHEK2 5
EGFR 123 BRAF 77 PRDM1 6
GNAQ 6 FGFR1 2 CDKN2A 108
KRAS 63 IDH2 12 FGFR3 17
PTPN11 28 NPM1 28 JAK3 6
CD28 3 SMO 5 PDGFRA 26
PLCG1 3 CDH1 7 STK11 22
ALK 8 FGFR2 8 CTNNB1 73
ERBB4 13 JAK2 5 GNA11 5
HNF1A 10 NRAS 35 KIT 139
MLH1 1 SRC 1 PTEN 146
RET 17 JAK1 12 VHL 124
APC 164 IL7R 4 CSF1R 8
EZH2 11 IL2RG 3 FLT3 30
TP53 1087 TNFAIP3 9 KDR 11
FYN 3 PIK3CA 97    


The above table shows 50 such targets along with COSMIC mutations associated with each of these genes (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978258/) - these genes were tiled 2x for higher coverage. FOXA1 and TERT promoter were tiled at 4x. All other regions were tiled at 1x, as per our standard practices.

With curated content from the leading canine cancer researchers in the world combined with Twist's robust panel performance, the Twist Alliance Canine Exome provides the most optimal tool for researchers studying canine diseases as well as rare human diseases.

1 Overgaard,N.H.,Fan,T.M.,Schachtschneider,K.M.,Principe,D.R.,Schook,L.B.,& Jungersen, G. (2018). Of Mice, Dogs, Pigs, and Men: Choosing the Appropriate Model for Immuno-Oncology Research. ILAR journal, 59(3), 247–262.

Note: The Twist Alliance Canine Exome - 40.5 MB is not ISO-13485 certified.

Ordering Information

105305

Twist Alliance Canine Exome - 40.5 Mb, 2 Reactions, Kit

105306

Twist Alliance Canine Exome - 40.5 Mb, 12 Reactions, Kit

105307

Twist Alliance Canine Exome - 40.5 Mb, 96 Reactions, Kit