Twist 96-Plex High Throughput NGS Library Prep for Genotyping in Populations

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Over the past decade, genotyping technology has transitioned from PCR-based SNP assays to microarrays and is now shifting toward high-throughput genotyping by sequencing (GBS). Here we present data on 96 Zea mays (maize) samples consisting of 4 parent populations and 92 recombinant inbred lines (RILs). The Twist 96-Plex Library Preparation Kit when combined with low coverage sequencing and imputation-based genotype analysis, provides NGS level information at a significantly reduced cost.

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High throughput library prep workflow

Utility of low coverage sequencing and imputation

Demonstration of low-cost haplotype marker, SNV, and SV data at scale

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Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Twist 96-Plex High Throughput NGS Library Prep for Genotyping in Populations

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