High-Throughput Antibody Discovery Targeting Cell Surface Receptors
Learn how we use high-throughput iQue flow cytometry to make more informed decisions during antibody screening.
HIGH-THROUGHPUT ANTIBODY SCREENING
Abveris provides high-throughput antibody screening services to discover, characterize, and deliver optimal monoclonal antibodies.
We go beyond testing for antibody specificity using our high-throughput technology platforms. We test delivered antibodies for desired binding kinetics, epitope specificity, on-cell cross-reactivity, and functional binding profiles.
INTELLICYT IQUE FLOW CYTOMETRY
With our iQue Screener PLUS flow cytometer we screen antibodies for on-cell binding to identify massive panels of antigen-specific antibodies for further interrogation.
Advantages of the IntelliCyt iQue PLUS
The iQue achieves faster assay throughput than traditional flow cytometers by sampling only microliters from each well and delivering an air-gap-delimited flow of samples to the detectors.
The iQue Screener PLUS converts the traditional low throughput, time-consuming flow cytometry approach into a high throughput antibody screening process.
The end result is a data-rich snapshot of the molecular events occurring in each well that can take less than five minutes for a 96-well plate.
QUESTIONS WE ANSWER WITH IQUE:
- on-cell binding
- affinity (apparent KD)
- functional antibodies
- species cross-reactivity
OCTET BLI & CARTERRA SPR
Real time label-free binding analyses have introduced a new era in antibody screening and characterization, quickly. Abveris employs Carterra Surface Plasmon Resonance (SPR) and Octet Bio-Layer Interferometry (BLI) for detection of label-free molecular interactions.
Carterra SPR and Octet BLI are fully integrated into our custom monoclonal antibody development so that we can screen hundreds of monoclonal antibody candidates for desired binding specificity and kinetics suited for your intended application(s).
THE ADVANTAGE OF HIGH-THROUGHPUT ANTIBODY SCREENING WITH ABVERIS:
- Species Cross-Reactivity Assessments
- Kinetics, Affinity, KD determination
- Epitope Binning, Antibody Pairing
- Ligand Blocking Analyses
- Rapid Antibody Quantitation
Both the Carterra and Octet-based analyses are stand-alone services available to partners interested in binding kinetic analyses.