How do I determine library mass into target capture?
The library mass used in target enrichment is calculated based on the cfDNA sample mass input into library preparation. Use the concentration of each amplified, indexed library to calculate the volume (in μl) of each library needed for hybridization when performing a singleplex or 8-plex.
This information can also be found in Appendix B of the protocol here: https://www.twistbioscience.com/resources/protocol/twist-cfdna-target-enrichment-stand ard-hybri dization-protocol
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