What is not included with the cfDNA Library Preparation Kit?
Users will need to procure an amplification mix kit. Twist recommends the HiFi HotStart ReadyMix from Roche/KAPA.
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NGS Kits
Twist cfDNA LP and Hyb Mix Kit
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Product Configuration
- What are the kit configurations of the cfDNA Library Preparation Kit?
- What are the shipping and storage conditions of the cfDNA Library Preparation Kit?
- What is the shelf life of the cfDNA Library Preparation Kit?
- Should I worry about freeze/thaw cycles with the cfDNA Library Prep Kit?
- What is not included with the cfDNA Library Preparation Kit?
Sample Input
- How much DNA input can I use with the cfDNA Library Preparation Kit?
- How do I determine how much cfDNA to use?
- Do you recommend any QC assays to perform on the input cfDNA prior to library preparation?
- Can I increase the volume of cfDNA input if my DNA concentration is very low?
- Can I use sample DNA other than cfDNA with this kit?
- Can I only use 1 - 20 ng with the DNA derived from FFPE samples?
Library Preparation
- What are the advantages of the cfDNA Library Preparation kit as compared to other kits?
- How long does it take to construct a library using the cfDNA Library Preparation Kit?
- What kind of sequencing libraries does the cfDNA Library Preparation Kit support?
- Does the cfDNA Library Preparation Kit support Unique Molecular Identifiers (UMIs)?
- What if I do not need the UMIs with the cfDNA Library Prep kit? Can I use UDI adapters?
- Can I adjust the SPRI ratio after ligation from the recommended 0.9X?
- Why do I see larger peaks in my cfDNA library amplification?
Hybridization
- What plexity does the cfDNA Target Enrichment Kits support?
- How do I determine library mass into target capture?
- How did you derive this 80x multiplier library input into target capture and do I need to follow it?
- How much mass can I add into a singleplex target enrichment
- What will happen if more than 12.8 μg input mass is added into capture?
- How do I set up 8-plex Target Enrichment with libraries made from different sample mass inputs?
- What is the recommendation if I have libraries made from sample mass inputs >20 ng?
