Methylated UMI Adapters
- What is the adapter structure of the methylated UMIs?
- How many bases are the UMI sequences?
- Are the UMI sequences available?
- Are the methylated UMIs compatible with NEB EM-seq?
- Do you have guidance on how to process sequencing data generated with the methylated UMIs?
- Are the methylated UMIs compatible with gDNA as well as cfDNA?
Library Preparation
- Does Twist offer library preparation kits that use enzymatic fragmentation?
- Does Twist offer library preparation kits that use mechanical fragmentation?
- What is the ratio of adapter to DNA fragments in the ligation step?
- If I use a non-Twist library prep method, what is the recommended size of DNA inserts?
- What complete kit configurations for NGS library preparation and enrichment does Twist offer?
- Can I use the Twist kits to make libraries for Whole Genome Sequencing?
- Are Twist adapters compatible with enzymatic and mechanical based fragmentation methods?
- Can you purchase individual components of the enrichment kits separately?
Methylation
- What method of fragmentation can I use in this workflow?
- What is the recommended insert size?
- What is the final library size?
- What is my expected final library yield?
- Are there any internal controls to test for methylation conversion?
- What is the min/max of DNA input for library preparation?
- Can I use the Standard Hybridization workflow for enrichment?
- Can I multiplex libraries in the capture; if so, what is the amount of library required?
- What modifications to the protocol can be made to optimize hybrid capture?
- What are the recommended number of amplification cycles post-capture?
- Should I use the Methylation Enhancer?
Target Enrichment
- Is there a maximum amount of DNA I can put into the hybridization? Can I add more than 1500 ng of DNA?
- What are the sequences of the Post-capture amplification primers in the NGS kit?
- What is the concentration of the amplification primers used in the post-enrichment PCR?
- Can I use Twist Universal Blockers with other adapters?
General
Twist 96-Plex LP Kit
- What are the kit sizes?
- Are there additional consumables and tools that the customer must supply?
- How do I order a kit?
- Can I order the individual components of the kit?
- What protocols are available?
- What is the shelf life of this kit?
- What sequencer should I use with this kit?
- Is it feasible to prepare less than 96 samples at once?
- What are the recommendations for DNA input, quality and quantity?
- Are unique dual indexes available with the kit?
- Do you have any recommendations for loading concentrations?
- How does the kit adapt to samples with varying GC content?
- What do you recommend as the demux tool?
- What sequences should we use for adaptor trimming of 96-Plex libraries?
- Can 96-Plex libraries be pooled for sequencing with dual indexed libraries?
- Is it possible to have only one plate of samples in a sequencing run?
- Are there any considerations for using patterned flowcells?
Twist cfDNA LP and Hyb Mix Kit
Product Configuration
- What are the kit configurations of the cfDNA Library Preparation Kit?
- What are the shipping and storage conditions of the cfDNA Library Preparation Kit?
- What is the shelf life of the cfDNA Library Preparation Kit?
- Should I worry about freeze/thaw cycles with the cfDNA Library Prep Kit?
- What is not included with the cfDNA Library Preparation Kit?
Sample Input
- How much DNA input can I use with the cfDNA Library Preparation Kit?
- How do I determine how much cfDNA to use?
- Do you recommend any QC assays to perform on the input cfDNA prior to library preparation?
- Can I increase the volume of cfDNA input if my DNA concentration is very low?
- Can I use sample DNA other than cfDNA with this kit?
- Can I only use 1 - 20 ng with the DNA derived from FFPE samples?
Library Preparation
- What are the advantages of the cfDNA Library Preparation kit as compared to other kits?
- How long does it take to construct a library using the cfDNA Library Preparation Kit?
- What kind of sequencing libraries does the cfDNA Library Preparation Kit support?
- Does the cfDNA Library Preparation Kit support Unique Molecular Identifiers (UMIs)?
- What if I do not need the UMIs with the cfDNA Library Prep kit? Can I use UDI adapters?
- Can I adjust the SPRI ratio after ligation from the recommended 0.9X?
- Why do I see larger peaks in my cfDNA library amplification?
Hybridization
- What plexity does the cfDNA Target Enrichment Kits?
- How do I determine library mass into target capture?
- How did you derive this 80x multiplier library input into target capture and do I need to follow it?
- How much mass can I add into a singleplex target enrichment
- What will happen if more than 12.8 μg input mass is added into capture?
- How do I set up 8-plex Target Enrichment with libraries made from different sample mass inputs?
- What is the recommendation if I have libraries made from sample mass inputs >20 ng?
