Twist TrueAmp Polymerase Mix

Twist TrueAmp Polymerase Mix is rated for up to 20 freeze/thaw cycles with no change in performance. The mix is stable for 2 years.

The Twist TrueAmp Polymerase mix is shipped and should be stored at –20°C.

We have demonstrated long-read sequencing performance comparable to current market leading polymerases and can be used as a drop-in replacement, but we are now pivoting our development to specialize exclusively in high-fidelity long-read solutions.

A hotstart aptamer works by forming a secondary structure at low temperatures (like RT or during setup on the benchtop). This secondary structure binds to the polymerase enzyme and inactivates the enzymatic activity. At higher temperatures (like during PCR thermalcycling) the aptamer’s secondary structure becomes denatured and releases hold of the enzyme.  

As part of our QC process we integrate a 10 hour incubation hold at 37 degree C to ensure low temperature setup does not affect performance. 

The Twist TrueAmp Polymerase Mix is compatible with a broad range of sample types and is designed to work with a variety of genomic inputs common in NGS research namely purified genomic DNA (gDNA), cell-free DNA (cfDNA) and Formalin-Fixed Paraffin- Embedded (FFPE) DNA.

The new Twist TrueAmp Polymerase mix is engineered and optimized for high yield and robust performance which allows it to efficiently amplify both low-input DNA down to 100 fg and standard inputs, providing a single, reliable solution across diverse sample types.

The Twist TrueAmp Polymerase Mix is for research use only.

Twist TrueAmp Polymerase Mix is rated for up to 20 freeze/thaw cycles with no change in performance. The mix is stable for 2 years.

The Twist TrueAmp Polymerase mix is shipped and should be stored at –20°C.

We have demonstrated long-read sequencing performance comparable to current market leading polymerases and can be used as a drop-in replacement, but we are now pivoting our development to specialize exclusively in high-fidelity long-read solutions.

A hotstart aptamer works by forming a secondary structure at low temperatures (like RT or during setup on the benchtop). This secondary structure binds to the polymerase enzyme and inactivates the enzymatic activity. At higher temperatures (like during PCR thermalcycling) the aptamer’s secondary structure becomes denatured and releases hold of the enzyme.  

As part of our QC process we integrate a 10 hour incubation hold at 37 degree C to ensure low temperature setup does not affect performance. 

The Twist TrueAmp Polymerase Mix is compatible with a broad range of sample types and is designed to work with a variety of genomic inputs common in NGS research namely purified genomic DNA (gDNA), cell-free DNA (cfDNA) and Formalin-Fixed Paraffin- Embedded (FFPE) DNA.

The new Twist TrueAmp Polymerase mix is engineered and optimized for high yield and robust performance which allows it to efficiently amplify both low-input DNA down to 100 fg and standard inputs, providing a single, reliable solution across diverse sample types.

The Twist TrueAmp Polymerase Mix is for research use only.