Select your Library prep
This tool has been designed to calculate master mix volumes for use during Enyzmatic Fragmentation Library prep Kit 1 with UDI adapters. To use, enter the desired number of reactions in the highlighted field below to calculate volumes necessary for each step of the library preparation.
IMPORTANT: This tool automatically incorporates 10% dead volume to compensate for transfer loss
EF1.0 + UDI
Enzymatic Fragmentation and Twist Universal Adapter System
EF2.0 + UDI
Enzymatic Fragmentation 2.0 and Twist Universal Adapter System
Mech LP + UDI
Mechanical Fragmentation Library Prep and Twist Universal Adapter System
Mech LP + UMI
Mechanical Fragmentation Library Prep and Twist Universal Adapter System
NEB EM-Seq Library Prep
NEBNext Enzymatic Methyl-seq Library Preparation Protocol
