OVERVIEW MONKEYPOX CONTROLS SARS-COV-2 CONTROLS RESPIRATORY VIRUS CONTROLS RESOURCES
Overview

Trusted, accurate and safe controls for
a wide-range of applications

The recent Coronavirus pandemic has led to the unprecedented need for assays for detecting the presence of SARS-CoV-2 and other respiratory viruses in a variety of sample types. 

Reproducible, Quality Results

Positive controls provide quality control measures for a wide range of applications from assay development to routine testing, Positive controls provide quality control measures for a wide range of applications from assay development to routine testing of samples with both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays.

Alternative to “Live Virus”

Controls based on viral nucleic acids extracted from infected organisms or from virus propagated in culture can potentially have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.

Flexible formats for your applications

Choose from Standard, Assay Ready, or Encapsuled Control formulations. The standard format provides the controls at high concentration in a frozen liquid formulation. The Assay Ready Controls are provided as a dried pellet that increases stability. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s The Encapsulated Control products leverage a metal capsule sealed around a desiccated pellet to enhance stability of the RNA controls.

Both the Assay Ready and the Encapsulated formulations are single use controls. The Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers. The enhanced stability of the Encapsulated controls enables extended shelf life and both ship and store at room temperature. Ambient shipping reduces cost and increases accessibility for researchers across the globe.

  Standard Controls Assay Ready Controls Encapsulated Controls
Biosafety Level 1 Level 1 Level 1
Storage Temperature: -70°C to -90°C Temperature: -20°C Ambient     
(4°C - 40°C)
Specification Range Approximately 1x10^6 copies/μL Approximately 2x10^6 copies/tube Approximately 50,000 copies/tube
Physical State Frozen liquid Dried pellet Encapsulated dried pellet
Shelf Life 2 years 2 years 5 years
Shipping Dry Ice Ambient Ambient 
Sensitive Detection
Fully synthetic RNA
Includes fully synthetic RNA generated from Twist gene fragments
Alternative to live viruses
Sequencing
Viral genome coverage
>99.9% coverage of target viral genome regions
NGS sequence verified
Enables versatility in assay design
Versatile controls
Versatile controls
Positive controls perform for both qPCR and NGS assays
Content is well-aligned with Twist target enrichment panels
New Control Variants Cover the Recent Strains That Impact the World

Now available: Variants of Interest and Variants of Concern, see table on SARS-CoV-2 Controls tab

•    Control 14 Alpha (B.1.1.7 lineage) 
•    Control 15 Alpha (B.1.1.7 lineage) 
•    Control 16 Beta (B.1.351 lineage) 
•    Control 17 Gamma (P.1 lineage) 
•    Control 18 Kappa (B.1.617.1 lineage) 
•    Control 19 Iota (B.1.526 lineage)
•    Control 21 Epsilon (B1.1.429 lineage)
•    Control 23 Delta (B.1.617.2 lineage) 
•    Control 24 (B.1.617.3 lineage) 
•    Control 28 Delta (AY.1 lineage) 
•    Control 29 Delta (AY.2 lineage)
•    Control 48 Omicron (B.1.1.529, BA.1 lineage)
•    Control 50 Omicron (B.1.1.529, BA.2 lineage) 
•    Control 51 Omicron (B.1.1.529, BA.2 lineage) 
•    Control 62 Omicron (B.2.12.1 lineage)
•    Control 63 Omicron (B.2.12.1 lineage)

TARGETED TOOLS FOR SARS-CoV-2 RESEARCH

Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.

Watch the Webinar

Trusted, accurate and safe controls for
a wide-range of applications

The recent Coronavirus pandemic has led to the unprecedented need for assays for detecting the presence of SARS-CoV-2 and other respiratory viruses in a variety of sample types. 

Reproducible, Quality Results

Positive controls provide quality control measures for a wide range of applications from assay development to routine testing, Positive controls provide quality control measures for a wide range of applications from assay development to routine testing of samples with both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays.

Alternative to “Live Virus”

Controls based on viral nucleic acids extracted from infected organisms or from virus propagated in culture can potentially have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.

Flexible formats for your applications

Choose from Standard, Assay Ready, or Encapsuled Control formulations. The standard format provides the controls at high concentration in a frozen liquid formulation. The Assay Ready Controls are provided as a dried pellet that increases stability. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s The Encapsulated Control products leverage a metal capsule sealed around a desiccated pellet to enhance stability of the RNA controls.

Both the Assay Ready and the Encapsulated formulations are single use controls. The Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers. The enhanced stability of the Encapsulated controls enables extended shelf life and both ship and store at room temperature. Ambient shipping reduces cost and increases accessibility for researchers across the globe.

  Standard Controls Assay Ready Controls Encapsulated Controls
Biosafety Level 1 Level 1 Level 1
Storage Temperature: -70°C to -90°C Temperature: -20°C Ambient     
(4°C - 40°C)
Specification Range Approximately 1x10^6 copies/μL Approximately 2x10^6 copies/tube Approximately 50,000 copies/tube
Physical State Frozen liquid Dried pellet Encapsulated dried pellet
Shelf Life 2 years 2 years 5 years
Shipping Dry Ice Ambient Ambient 
Sensitive Detection
Fully synthetic RNA
Includes fully synthetic RNA generated from Twist gene fragments
Alternative to live viruses
Sequencing
Viral genome coverage
>99.9% coverage of target viral genome regions
NGS sequence verified
Enables versatility in assay design
Versatile controls
Versatile controls
Positive controls perform for both qPCR and NGS assays
Content is well-aligned with Twist target enrichment panels
New Control Variants Cover the Recent Strains That Impact the World

Now available: Variants of Interest and Variants of Concern, see table on SARS-CoV-2 Controls tab

•    Control 14 Alpha (B.1.1.7 lineage) 
•    Control 15 Alpha (B.1.1.7 lineage) 
•    Control 16 Beta (B.1.351 lineage) 
•    Control 17 Gamma (P.1 lineage) 
•    Control 18 Kappa (B.1.617.1 lineage) 
•    Control 19 Iota (B.1.526 lineage)
•    Control 21 Epsilon (B1.1.429 lineage)
•    Control 23 Delta (B.1.617.2 lineage) 
•    Control 24 (B.1.617.3 lineage) 
•    Control 28 Delta (AY.1 lineage) 
•    Control 29 Delta (AY.2 lineage)
•    Control 48 Omicron (B.1.1.529, BA.1 lineage)
•    Control 50 Omicron (B.1.1.529, BA.2 lineage) 
•    Control 51 Omicron (B.1.1.529, BA.2 lineage) 
•    Control 62 Omicron (B.2.12.1 lineage)
•    Control 63 Omicron (B.2.12.1 lineage)

TARGETED TOOLS FOR SARS-CoV-2 RESEARCH

Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.

Watch the Webinar

Monkeypox Controls
Monkeypox Controls

Twist’s Synthetic human Monkeypox Virus (hMPXV) Controls are based on sequences from either the Congo Basin (CB) or West African (WA) clades of hMPXV and provide coverage of ≥80% of the viral genome. As a result, these controls support the design of custom assays targeting regions of the genome relevant to orthopoxvirus research and can be used for both amplicon and capture-based detection methods. Twist’s hMPXV controls have been validated and are compatible with CDC recommended real-time PCR testing procedures for monkeypox detection. Control 1 and Control 2 are composed of 154 and 151 overlapping 1.8kb fragments, respectively, which tile the genome at an interval of 900bp. Each control is present at  copies and provided at a concentration of approximately 100,000 copies/uL. The figure on the right shows a taxonomic relationship between the WA and CB clades of hMPXV as well as other orthopoxviruses. Please contact your local sales representative for more information.

Note: The Twist Synthetic human Monkeypox Virus
(hMPXV) Controls are not ISO-13485 certified

 

Ortho
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls

Related Resources

table
Monkeypox Controls

Twist’s Synthetic human Monkeypox Virus (hMPXV) Controls are based on sequences from either the Congo Basin (CB) or West African (WA) clades of hMPXV and provide coverage of ≥80% of the viral genome. As a result, these controls support the design of custom assays targeting regions of the genome relevant to orthopoxvirus research and can be used for both amplicon and capture-based detection methods. Twist’s hMPXV controls have been validated and are compatible with CDC recommended real-time PCR testing procedures for monkeypox detection. Control 1 and Control 2 are composed of 154 and 151 overlapping 1.8kb fragments, respectively, which tile the genome at an interval of 900bp. Each control is present at  copies and provided at a concentration of approximately 100,000 copies/uL. The figure on the right shows a taxonomic relationship between the WA and CB clades of hMPXV as well as other orthopoxviruses. Please contact your local sales representative for more information.

Note: The Twist Synthetic human Monkeypox Virus
(hMPXV) Controls are not ISO-13485 certified

 

Ortho
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls

Related Resources

table
SARS-CoV-2 Controls
The use of positive controls in quality control

Twist has created synthetic RNA controls for several viral genomes. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome. The majority of the controls are provided in the standard format which consist of 100 μL at a concentration of approximately one million copies per microliter. Twist also offers certain controls in Assay Ready and Encapsulated Formats that are supplied as a desiccated pellet. The Assay Ready Controls are supplied at approximately two million copies per tube. The Encapsulated Controls are supplied at approximately 50,000 copies per tube. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control was developed in partnership with the US CDC and integrated into the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which tests for influenza A, B and SARS-CoV-2 simultaneously. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control is now available for customers outside the CDC. 

Twist Bioscience Encapsulated Control products offer a unique storage and delivery method for Twist’s established Synthetic SARS-CoV-2 RNA controls. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s Encapsulated Control products leverage a novel method to seal a metal capsule around a desiccated pellet to enhance stability of the RNA controls. Due to the enhanced stability, they can be shipped and stored at room temperature, reducing cost and improving accessibility globally. The Encapsulated Controls are sold in a rack of 16 or 96 tubes, are single use, and have a 5-year shelf life from the date of manufacture.

Available SARS-CoV-2 Controls

Best practices for using Assay Ready and Encapsulated Controls

For the Storage and use of Assay Ready or Encapsulated SARS-CoV-2 Controls please review the Following documents:


This product has not been FDA cleared or approved, but has been authorized for emergency use by FDA under an EUA for use by authorized laboratories; This product has been authorized only for the detection of nucleic acid from SARS-CoV-2, along with the identification and differentiation of SARS-CoV-2 PANGO lineages and the identification of specific SARS-CoV-2 genomic mutations, not for any other viruses or pathogens; and, the emergency use of this product is only authorized for the duration of the declaration that circumstances exist justifying the authorization of emergency use of in vitro diagnostics for detection and/or diagnosis of COVID-19 under Section 564(b)(1) of the Federal Food, Drug and Cosmetic Act, 21 U.S.C. § 360bbb-3(b)(1), unless the declaration is terminated or authorization is revoked sooner.

NGS SARS-CoV-2 Synthetic RNA Controls Genome

 

 

PART # CONTROL GenBank/GISAID ID GISAID NAME
102019 Control 1 MT007544.1 Australia/VIC01/2020
102024 Control 2 MN908947.3 Wuhan-Hu-1
103925 Assay Ready Control 2 MN908947.3 Wuhan-Hu-1
104422 Encapsulated Control 2 (16) MN908947.3 Wuhan-Hu-1
104426 Encapsulated Control 2 (96) MN908947.3 Wuhan-Hu-1
102860 Control 3 LC528232.1 Japan/Hu_DP_Kng_19-020/2020
102862 Control 4 MT106054.1 USA/TX1/2020
102917 Control 5 MT188340 USA/MN2-MDH2/2020
102918 Control 6 MT118835 USA/CA9/2020
103087 Control 7 EPI_ISL_418227 France/HF2393/2020
103511 Control 8 MT066176 Taiwan/NTU02/2020
103512 Control 9 MT152824 USA/WA2/2020
103513 Control 10 EPI_ISL_414648 USA/CA-PC101P/2020
103514 Control 11 EPI_ISL_417739 Iceland/5/2020
103515 Control 12 EPI_ISL_420244 England/SHEF-C05B2/2020
103533 Control 13 EPI_ISL_421184 Belgium/ULG-10004/2020
103907 Control 14
Alpha
EPI_ISL_710528 England/205041766/2020
103926 Assay Ready Control 14
Alpha (B.1.1.7)
EPI_ISL_710528 England/205041766/2020
103909 Control 15
Alpha
EPI_ISL_601443 England/MILK-9E05B3/2020
103927

Assay Ready Control 15
Alpha (B.1.1.7)

EPI_ISL_601443 England/MILK-9E05B3/2020
104043 Control 16
Beta (B.1.351)
EPI_ISL_678597 South Africa/KRISP-EC-K005299/2020
104044 Control 17
Gamma (P.1)
EPI_ISL_792683 Japan (Brazil) /IC-0564/2021
104338

Control 18
Kappa (B.1.617.1)

EPI_ISL_1662307 India/CT-ILSGS00361/2021
104529

Control 19
Iota (B.1.526)

EPI_ISL_1300881 USA/NY-MSHSPSP-PV24650/2020
104530 Control 20 (B.1.427) EPI_ISL_730092 USA/CA-ALSR-4704/2020
104531

Control 21
Epsilon (B.1.429)

EPI_ISL_672365 USA/CA-CZB-12943/2020
104532 Control 22 (B1.1.519) EPI_ISL_933685 Mexico/CMX-InDRE_208/2021

104533

Control 23
Delta (B.1.617.2)
EPI_ISL_1544014 India/MH-NCCS-P1162000182735/2021
104534 Control 24
(B.1.617.3)
EPI_ISL_1939891 India/MH-SEQ-221_S66_R1_001/2021
104538

Control 28  
Delta (AY.1)

EPI_ISL_2695467 Portugal/PT9543/2021
104423 Encapsulated Control 28 (16) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104427 Encapsulated Control 28 (96) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104539

Control 29  
Delta (AY.2)

EPI_ISL_2693246 USA/WA-CDC-UW21061750277/2021
105204 Control 48 (B.1.1.529/BA.1) EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104424 Encapsulated Control 48 (16)
(B.1.1.529/BA.1)
EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104428 Encapsulated Control 48 (96)
(B.1.1.529/BA.1)
EPI_ISL_6841980

Hong Kong/HKU-211129-001/2021

105345 Control 50 (B.1.1.529+BA.2) EPI_ISL_7190366 Australia/QLD2568/2021
105346 Control 51 (B.1.1.529+BA.2) EPI_ISL_7718520 England/MILK-2DF642C/2021
105865 Control 62 (B.2.12.1) EPI_ISL_12248637.1 Denmark/DCGC-493190/2022
105857 Control 63 (B.2.12.1)

 EPI_ISL_12303256.1

USA/NY-CDC-LC0579415/2022
106196 Control 64 (BA.5) EPI_ISL_12516495 hCoV-19/England/LSPA-3DC1269/2022
106197 Control 65 (BA.5) EPI_ISL_12620611 hCoV-19/USA/TN-ASC-210769476/2022
106198 Control 66 (BA.4) EPI_ISL_12454576 hCoV-19/USA/TX-HMH-M-96682/2022
106199 Control 67 (BA.4) EPI_ISL_12605687 hCoV-19/USA/CA-CDC-QDX36065390/202

106929

Control 70

EPI_ISL_14829147

hCoV-19/USA/MI-CDC-QDX40579621/2022

106930

Control 71

EPI_ISL_15381979

hCoV-19/Australia/SA464919/2022

 

 

The use of positive controls in quality control

Twist has created synthetic RNA controls for several viral genomes. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome. The majority of the controls are provided in the standard format which consist of 100 μL at a concentration of approximately one million copies per microliter. Twist also offers certain controls in Assay Ready and Encapsulated Formats that are supplied as a desiccated pellet. The Assay Ready Controls are supplied at approximately two million copies per tube. The Encapsulated Controls are supplied at approximately 50,000 copies per tube. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control was developed in partnership with the US CDC and integrated into the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which tests for influenza A, B and SARS-CoV-2 simultaneously. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control is now available for customers outside the CDC. 

Twist Bioscience Encapsulated Control products offer a unique storage and delivery method for Twist’s established Synthetic SARS-CoV-2 RNA controls. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s Encapsulated Control products leverage a novel method to seal a metal capsule around a desiccated pellet to enhance stability of the RNA controls. Due to the enhanced stability, they can be shipped and stored at room temperature, reducing cost and improving accessibility globally. The Encapsulated Controls are sold in a rack of 16 or 96 tubes, are single use, and have a 5-year shelf life from the date of manufacture.

Available SARS-CoV-2 Controls

Best practices for using Assay Ready and Encapsulated Controls

For the Storage and use of Assay Ready or Encapsulated SARS-CoV-2 Controls please review the Following documents:


This product has not been FDA cleared or approved, but has been authorized for emergency use by FDA under an EUA for use by authorized laboratories; This product has been authorized only for the detection of nucleic acid from SARS-CoV-2, along with the identification and differentiation of SARS-CoV-2 PANGO lineages and the identification of specific SARS-CoV-2 genomic mutations, not for any other viruses or pathogens; and, the emergency use of this product is only authorized for the duration of the declaration that circumstances exist justifying the authorization of emergency use of in vitro diagnostics for detection and/or diagnosis of COVID-19 under Section 564(b)(1) of the Federal Food, Drug and Cosmetic Act, 21 U.S.C. § 360bbb-3(b)(1), unless the declaration is terminated or authorization is revoked sooner.

NGS SARS-CoV-2 Synthetic RNA Controls Genome

 

 

PART # CONTROL GenBank/GISAID ID GISAID NAME
102019 Control 1 MT007544.1 Australia/VIC01/2020
102024 Control 2 MN908947.3 Wuhan-Hu-1
103925 Assay Ready Control 2 MN908947.3 Wuhan-Hu-1
104422 Encapsulated Control 2 (16) MN908947.3 Wuhan-Hu-1
104426 Encapsulated Control 2 (96) MN908947.3 Wuhan-Hu-1
102860 Control 3 LC528232.1 Japan/Hu_DP_Kng_19-020/2020
102862 Control 4 MT106054.1 USA/TX1/2020
102917 Control 5 MT188340 USA/MN2-MDH2/2020
102918 Control 6 MT118835 USA/CA9/2020
103087 Control 7 EPI_ISL_418227 France/HF2393/2020
103511 Control 8 MT066176 Taiwan/NTU02/2020
103512 Control 9 MT152824 USA/WA2/2020
103513 Control 10 EPI_ISL_414648 USA/CA-PC101P/2020
103514 Control 11 EPI_ISL_417739 Iceland/5/2020
103515 Control 12 EPI_ISL_420244 England/SHEF-C05B2/2020
103533 Control 13 EPI_ISL_421184 Belgium/ULG-10004/2020
103907 Control 14
Alpha
EPI_ISL_710528 England/205041766/2020
103926 Assay Ready Control 14
Alpha (B.1.1.7)
EPI_ISL_710528 England/205041766/2020
103909 Control 15
Alpha
EPI_ISL_601443 England/MILK-9E05B3/2020
103927

Assay Ready Control 15
Alpha (B.1.1.7)

EPI_ISL_601443 England/MILK-9E05B3/2020
104043 Control 16
Beta (B.1.351)
EPI_ISL_678597 South Africa/KRISP-EC-K005299/2020
104044 Control 17
Gamma (P.1)
EPI_ISL_792683 Japan (Brazil) /IC-0564/2021
104338

Control 18
Kappa (B.1.617.1)

EPI_ISL_1662307 India/CT-ILSGS00361/2021
104529

Control 19
Iota (B.1.526)

EPI_ISL_1300881 USA/NY-MSHSPSP-PV24650/2020
104530 Control 20 (B.1.427) EPI_ISL_730092 USA/CA-ALSR-4704/2020
104531

Control 21
Epsilon (B.1.429)

EPI_ISL_672365 USA/CA-CZB-12943/2020
104532 Control 22 (B1.1.519) EPI_ISL_933685 Mexico/CMX-InDRE_208/2021

104533

Control 23
Delta (B.1.617.2)
EPI_ISL_1544014 India/MH-NCCS-P1162000182735/2021
104534 Control 24
(B.1.617.3)
EPI_ISL_1939891 India/MH-SEQ-221_S66_R1_001/2021
104538

Control 28  
Delta (AY.1)

EPI_ISL_2695467 Portugal/PT9543/2021
104423 Encapsulated Control 28 (16) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104427 Encapsulated Control 28 (96) Delta (AY.1) EPI_ISL_2695467 Portugal/PT9543/2021
104539

Control 29  
Delta (AY.2)

EPI_ISL_2693246 USA/WA-CDC-UW21061750277/2021
105204 Control 48 (B.1.1.529/BA.1) EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104424 Encapsulated Control 48 (16)
(B.1.1.529/BA.1)
EPI_ISL_6841980 Hong Kong/HKU-211129-001/2021
104428 Encapsulated Control 48 (96)
(B.1.1.529/BA.1)
EPI_ISL_6841980

Hong Kong/HKU-211129-001/2021

105345 Control 50 (B.1.1.529+BA.2) EPI_ISL_7190366 Australia/QLD2568/2021
105346 Control 51 (B.1.1.529+BA.2) EPI_ISL_7718520 England/MILK-2DF642C/2021
105865 Control 62 (B.2.12.1) EPI_ISL_12248637.1 Denmark/DCGC-493190/2022
105857 Control 63 (B.2.12.1)

 EPI_ISL_12303256.1

USA/NY-CDC-LC0579415/2022
106196 Control 64 (BA.5) EPI_ISL_12516495 hCoV-19/England/LSPA-3DC1269/2022
106197 Control 65 (BA.5) EPI_ISL_12620611 hCoV-19/USA/TN-ASC-210769476/2022
106198 Control 66 (BA.4) EPI_ISL_12454576 hCoV-19/USA/TX-HMH-M-96682/2022
106199 Control 67 (BA.4) EPI_ISL_12605687 hCoV-19/USA/CA-CDC-QDX36065390/202

106929

Control 70

EPI_ISL_14829147

hCoV-19/USA/MI-CDC-QDX40579621/2022

106930

Control 71

EPI_ISL_15381979

hCoV-19/Australia/SA464919/2022

 

 

Respiratory Virus Controls
Available Respiratory Virus Controls

The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design. 

This figure shows a select taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.

NGS Respiratory Viral Controls Taxonomic Tree
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.

Contact your local sales representative for more details

or ORDER NOW

Part Number Name Accession Virus Type Length (bases)
103001 Twist Synthetic Influenza H1N1 (2009) RNA control NC_026431, NC_026432,
NC_026433, NC_026434,
NC_026435, NC_026436,
NC_026437, NC_026438
ssRNA (- sense) 13158
103002 Temperature: Twist Synthetic Influenza H3N2 RNA control NC_007366, NC_007367,
NC_007368, NC_007369,
NC_007370, NC_007371,
NC_007372, NC_007373
ssRNA (- sense) 14452
103003 Twist Synthetic Influenza B RNA control NC_002204, NC_002205,
NC_002206, NC_002207,
NC_002208, NC_002209,
NC_002210, NC_002211
ssRNA (- sense) 14452
103004 Twist Synthetic Human bocavirus 1 DNA control MG953830.1 ssDNA 5164
103005 Twist Synthetic Human bocavirus 1 DNA control NC_038308.1 ssRNA (+ sense) 7367
103006 Twist Synthetic Human rhinovirus 89 RNA control NC_001617.1 ssRNA (+ sense) 7152
103007 Twist Synthetic Mumps virus RNA control NC_002200.1 ssRNA (- sense) 15384
103008 Twist Synthetic Human parainfluenza virus 1 RNA control NC_003461.1 ssRNA (- sense) 15600
103009 Twist Synthetic Measles virus RNA control NC_001498.1 ssRNA (- sense) 15894
103010 Twist Synthetic Human parainfluenza virus 4 RNA control NC_021928.1 ssRNA (- sense) 17052
103011 Twist Synthetic Human coronavirus 229E RNA control NC_002645.1 ssRNA (+ sense) 27317
103012 Twist Synthetic Human coronavirus NL63 RNA control NC_005831.2 ssRNA (+ sense) 27553
103013 Twist Synthetic Human coronavirus OC43 RNA control NC_006213.1 ssRNA (+ sense) 30741
103730 Twist Respiratory Virus Controls (13 Count) N/A N/A N/A
Available Respiratory Virus Controls

The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design. 

This figure shows a select taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.

NGS Respiratory Viral Controls Taxonomic Tree
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.

Contact your local sales representative for more details

or ORDER NOW

Part Number Name Accession Virus Type Length (bases)
103001 Twist Synthetic Influenza H1N1 (2009) RNA control NC_026431, NC_026432,
NC_026433, NC_026434,
NC_026435, NC_026436,
NC_026437, NC_026438
ssRNA (- sense) 13158
103002 Temperature: Twist Synthetic Influenza H3N2 RNA control NC_007366, NC_007367,
NC_007368, NC_007369,
NC_007370, NC_007371,
NC_007372, NC_007373
ssRNA (- sense) 14452
103003 Twist Synthetic Influenza B RNA control NC_002204, NC_002205,
NC_002206, NC_002207,
NC_002208, NC_002209,
NC_002210, NC_002211
ssRNA (- sense) 14452
103004 Twist Synthetic Human bocavirus 1 DNA control MG953830.1 ssDNA 5164
103005 Twist Synthetic Human bocavirus 1 DNA control NC_038308.1 ssRNA (+ sense) 7367
103006 Twist Synthetic Human rhinovirus 89 RNA control NC_001617.1 ssRNA (+ sense) 7152
103007 Twist Synthetic Mumps virus RNA control NC_002200.1 ssRNA (- sense) 15384
103008 Twist Synthetic Human parainfluenza virus 1 RNA control NC_003461.1 ssRNA (- sense) 15600
103009 Twist Synthetic Measles virus RNA control NC_001498.1 ssRNA (- sense) 15894
103010 Twist Synthetic Human parainfluenza virus 4 RNA control NC_021928.1 ssRNA (- sense) 17052
103011 Twist Synthetic Human coronavirus 229E RNA control NC_002645.1 ssRNA (+ sense) 27317
103012 Twist Synthetic Human coronavirus NL63 RNA control NC_005831.2 ssRNA (+ sense) 27553
103013 Twist Synthetic Human coronavirus OC43 RNA control NC_006213.1 ssRNA (+ sense) 30741
103730 Twist Respiratory Virus Controls (13 Count) N/A N/A N/A
RESOURCES
Reproducible. Accurate. Safe.
Reproducible. Accurate. Safe.
Reproducible. Accurate. Safe.