Select your Library prep

EF1.0 + UDI

Enzymatic Fragmentation and Twist Universal Adapter System

EF2.0 + UDI

Enzymatic Fragmentation 2.0 and Twist Universal Adapter System

Mech LP + UDI

Mechanical Fragmentation Library Prep and Twist Universal Adapter System

Mech LP + UMI

Mechanical Fragmentation Library Prep and Twist Universal Adapter System

NEB EM-Seq Library Prep

NEBNext Enzymatic Methyl-seq Library Preparation Protocol

EF1.0 + UDI

Enzymatic Fragmentation and Twist Universal Adapter System

Reagent Single Reaction (uL) Total Volume (uL)
Enzymatic Fragmentation Master Mix
Water (chilled) 25
10x Fragmentation Buffer 5
5x Fragmentation Enzyme 10
Reaction Mix 40
Sample 10
Total (Frag/AT rxn) 50
Ligation Master Mix
Water (chilled) 15
DNA Ligation Buffer 20
DNA Ligation Mix 10
Reaction Mix 45
Twist Universal Adapter 5
Frag/AT rxn 50
Total 100
Twist UDI Indexing PCR
Equinox Library Amp Mix (2x) 25
Twist UDI Primer 10
Reaction Mix 35
Sample 15
Total 50
How to

This tool has been designed to calculate master mix volumes for use during Enyzmatic ...

This tool has been designed to calculate master mix volumes for use during Enyzmatic Fragmentation Library prep Kit 1 with UDI adapters. To use, enter the desired number of reactions in the highlighted field below to calculate volumes necessary for each step of the library preparation.

IMPORTANT: This tool automatically incorporates 10% dead volume to compensate for transfer loss

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