Generate 99% of Desired Variants
Protein engineering screens using single site variant libraries allow researchers to explore a protein’s sequence space and investigate the relationship between sequence and protein structure and function.
Twist Site Saturation Variant Library construction leverages massively parallel oligonucleotide synthesis using Twist's proprietary silicon-based DNA synthesis platform. Twist libraries are NGS-verified to confirm that all desired variants are present in the correct ratios.
Start Designing Your Library Now - A team of experts is waiting to help you design your perfect library for your specific needs. Order and Design here.
Learn more about how Twist is helping drug developers characterize and catalog mutations in KRAS through large-scale saturation mutagenesis screens.
Learn more about how Twist Single Site Variant Libraries (SSVL) enable the interrogation of sequence space to identify key residues in protein structure and function.
Generate 99% of Desired Variants
Protein engineering screens using single site variant libraries allow researchers to explore a protein’s sequence space and investigate the relationship between sequence and protein structure and function.
Twist Site Saturation Variant Library construction leverages massively parallel oligonucleotide synthesis using Twist's proprietary silicon-based DNA synthesis platform. Twist libraries are NGS-verified to confirm that all desired variants are present in the correct ratios.
Learn more about how Twist is helping drug developers characterize and catalog mutations in KRAS through large-scale saturation mutagenesis screens.
Learn more about how Twist Single Site Variant Libraries (SSVL) enable the interrogation of sequence space to identify key residues in protein structure and function.
Start Designing Your Library Now - A team of experts is waiting to help you design your perfect library for your specific needs. Order and Design here.
Site Saturation Libraries eliminate codon bias and unwanted substitutions unlike traditional methods such as degenerate and NNK approaches.
TRIM offers a poor repetitive yield, leading to <50% full-length product in a typical library whereas, Twist libraries yield more usable variants, increasing effective library size.
| Error Prone PCR | Degenerate
(NNK/NNS) |
Twist Site Saturation Variant Libraries |
|
|---|---|---|---|
| Eliminates sequence bias | No | No | Yes |
| Number of codons available | Unknown | 32 | All 64 |
| Prevents undesirable motifs | No | No | Yes |
| Allows codon optimization | No | No | Yes |
| Avoids stop codons | No | Yes | Yes |
Site Saturation Variant Libraries enable efficient sampling of a protein’s sequence space in screening assays.
This figure shows the observed distribution of amino acids across 65 positions in protein (19 intended variants per position) when built with Twist SSVLs versus error prone PCR. The bars represent a different amino acid position, with each color indicating the observed variant frequency. All variants are present in the expected ratios in the Twist Library.
Site Saturation Libraries eliminate codon bias and unwanted substitutions unlike traditional methods such as degenerate and NNK approaches.
TRIM offers a poor repetitive yield, leading to <50% full-length product in a typical library whereas, Twist libraries yield more usable variants, increasing effective library size.
| Error Prone PCR | Degenerate
(NNK/NNS) |
Twist Site Saturation Variant Libraries |
|
|---|---|---|---|
| Eliminates sequence bias | No | No | Yes |
| Number of codons available | Unknown | 32 | All 64 |
| Prevents undesirable motifs | No | No | Yes |
| Allows codon optimization | No | No | Yes |
| Avoids stop codons | No | Yes | Yes |
Site Saturation Variant Libraries enable efficient sampling of a protein’s sequence space in screening assays.
This figure shows the observed distribution of amino acids across 65 positions in protein (19 intended variants per position) when built with Twist SSVLs versus error prone PCR. The bars represent a different amino acid position, with each color indicating the observed variant frequency. All variants are present in the expected ratios in the Twist Library.
Let's Get Started
- Step 1: Complete the contact details form on this page
- Step 2: Download the SSVL Submission Form and fill out all fields in the form for your library design
- Step 3: Upload the completed submission form to the "Submit File" tab on this page
Ready to Submit?
- Step 1: Upload completed submission form
- Step 2: Our Library Team Experts will review your project
- Step 3: Library Team verifies the project and will reach out to you with a quote
- Step 4: Once the quote is accepted by the customer, Library Team will send the project to the production team
If you have any questions, please feel free to email us at [email protected]
Let's Get Started
- Step 1: Complete the contact details form on this page
- Step 2: Download the SSVL Submission Form and fill out all fields in the form for your library design
- Step 3: Upload the completed submission form to the "Submit File" tab on this page
Ready to Submit?
- Step 1: Upload completed submission form
- Step 2: Our Library Team Experts will review your project
- Step 3: Library Team verifies the project and will reach out to you with a quote
- Step 4: Once the quote is accepted by the customer, Library Team will send the project to the production team
If you have any questions, please feel free to email us at [email protected]
