Library Preparation

Yes, the Twist Library Prep kits are suitable to make libraries for whole genome sequencing.

As most WGS applications use longer read lengths (2x151 for Whole Genome Sequencing as opposed to 2x101 or 2x76 for Whole Exome Sequencing), the fragmentation conditions need to be changed to accommodate longer fragment lengths.

For the Enzymatic Fragmentation kits, length is dependent on three factors: Time, Temperature, and DNA input.

For Mechanical fragmentation, conditions (time, power, duty factor, and cycles per burst) should to be set to accommodate longer fragment lengths.

Yes, in addition to our Enzymatic Fragmentation kits, Twist also offers the following library preparation kits for use with Mechanical Fragmentation:

100875: Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples (includes enzymes and buffers)

101280: Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples (includes enzymes, buffers, and purification beads)

100876: Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples (includes enzymes and buffers)

101281:  Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples (includes enzymes, buffers, and purification beads)

Note for library preparation, you will also need adapters:

101307: Twist Universal Adapter System, 16 Samples

101308: Twist Universal Adapter System, 96 Samples – Plate A

101309: Twist Universal Adapter System, 96 Samples – Plate B

101310: Twist Universal Adapter System, 96 Samples – Plate C

101311: Twist Universal Adapter System, 96 Samples – Plate D

Assuming an input of 50 ng of DNA, the ratio of adapter to DNA is approximately 200:1.

Yes, Twist sells NGS kit components to allow for maximum flexibility.  You can obtain a complete list of orderable components by logging into your Twist Account.  You can order the following components:

 Library Prep Components      

• PN 100253 – TWIST LIBRARY PREP EF KIT 1, 16 SAMPLES    
• PN 100875 – TWIST LIBRARY PREP MF KIT 1, 16 SAMPLES   
• PN 100401 – TWIST LIBRARY PREP KIT 2, 16 SAMPLES                     
• PN 100572 – TWIST LIBRARY PREP EF KIT 1, 96 SAMPLES    
• PN 100876 – TWIST LIBRARY PREP MF KIT 1, 96 SAMPLES   
• PN 100573 – TWIST LIBRARY PREP KIT 2, 96 SAMPLES                     

Combinatorial Adapters        

• PN 100255 – 16 SAMPLES 

Unique Dual Indexed Adapters         

• PN 101307 – TWIST UNIVERSAL ADAPTER SYSTEM, 16 SAMPLES    
• PN 101308 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE A           
• PN 101309 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE B
• PN 101310 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE C
• PN 101311 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE D

Blockers         

• PN 100856 – TWIST UNIVERSAL BLOCKERS, 2 REACTIONS   
• PN 100578 – TWIST UNIVERSAL BLOCKERS, 12 REACTIONS 
• PN 100767 – TWIST UNIVERSAL BLOCKERS, 96 REACTIONS             

*Hybridization Reagents         

• PN 101263 – HYBRIDIZATION REAGENTS, 2 REACTIONS      
• PN 100930 – HYBRIDIZATION REAGENTS, 12 REACTIONS    
• PN 100982 – HYBRIDIZATION REAGENTS, 96 REACTIONS    

*Fast Hybridization Reagents  

• PN 101260 – FAST HYBRIDIZATION REAGENTS, 2 REACTIONS         
• PN 100964 – FAST HYBRIDIZATION REAGENTS, 12 REACTIONS       
• PN 100968 – FAST HYBRIDIZATION REAGENTS, 96 REACTIONS       

*Wash Buffers 

• PN 101264 – WASH BUFFERS, 2 REACTIONS 
• PN 100985 – WASH BUFFERS, 12 REACTIONS          
• PN 100846 – WASH BUFFERS, 96 REACTIONS          

*Fast Wash Buffers      

• PN 101261 – FAST WASH BUFFERS, 2 REACTIONS    
• PN 100971 – FAST WASH BUFFERS, 12 REACTIONS  
• PN 100972 – FAST WASH BUFFERS, 96 REACTIONS  

Purification and Binding Beads          

• PN 101262 – PURIFICATION AND STREPTAVIDIN BEADS, 2 REACTIONS      
• PN 100983 – PURIFICATION AND STREPTAVIDIN BEADS, 12 REACTIONS    
• PN 100984 – PURIFICATION AND STREPTAVIDIN BEADS, 96 REACTIONS   

*Note:  Standard Hybridization reagents should only be used with Standard Wash Buffers; conversely, Fast Hybridization reagents should only be used with Fast Wash Buffers.  Mixing Standard Hybridization and Fast Wash Buffers (and vice versa) will result in poor performance. 

Yes, Twist adapters are compatible with DNA fragments generated by either enzymatic or mechanical fragmentation.

Yes, in addition to our Mechanical Fragmentation kits, Twist also offers the following library preparation kits for use with Enzymatic Fragmentation:

100253: Twist Library Preparation Kit, Enzymatic Fragmentation, 16 Samples (includes enzymes and buffers)

101059: Twist Library Preparation Kit, Enzymatic Fragmentation, 16 Samples (includes enzymes, buffers, and purification beads)

100572: Twist Library Preparation Kit, Enzymatic Fragmentation, 96 Samples (includes enzymes and buffers)

101058:  Twist Library Preparation Kit, Enzymatic Fragmentation, 96 Samples (includes enzymes, buffers, and purification beads)

Note for library preparation, you will also need adapters:

101307: Twist Universal Adapter System, 16 Samples

101308: Twist Universal Adapter System, 96 Samples – Plate A

101309: Twist Universal Adapter System, 96 Samples – Plate B

101310: Twist Universal Adapter System, 96 Samples – Plate C

101311: Twist Universal Adapter System, 96 Samples – Plate D

Twist offers complete* kits for both Enzymatic Fragmentation and Mechanical Fragmentation - note these kits do not include an enrichment panel:

Enzymatic Fragmentation            

• PN 101897 – TWIST EF LIBRARY PREP + UDI AND STANDARD HYB, 16x2 REACTIONS          
• PN 101896 – TWIST EF LIBRARY PREP + UDI AND FAST HYB, 16x2 REACTIONS     
• PN 101901 – TWIST EF LIBRARY PREP + UDI AND STANDARD HYB, 96x12 REACTIONS       
• PN 101900 – TWIST EF LIBRARY PREP + UDI AND FAST HYB, 96x12 REACTIONS    

Mechanical Fragmentation        

• PN 101899 – TWIST MF LIBRARY PREP + UDI AND STANDARD HYB, 16x2 REACTIONS       
• PN 101898 – TWIST MF LIBRARY PREP + UDI AND FAST HYB, 16x2 REACTIONS    
• PN 101903 – TWIST MF LIBRARY PREP + UDI AND STANDARD HYB, 96x12 REACTIONS     
• PN 101902 – TWIST MF LIBRARY PREP + UDI AND FAST HYB, 96x12 REACTIONS

* Note: you must purchase KAPA® HiFi HotStart ReadyMix (Kapa Biosystems®, #KK2601) or its equivalent to generate and amplify libraries.

The recommended average target fragment size is 200 bp; this will result in an average library size of approximately 350 bp; however, you should adjust your insert size according to your sequencing read lengths.  

Yes, the Twist Library Prep kits are suitable to make libraries for whole genome sequencing.

As most WGS applications use longer read lengths (2x151 for Whole Genome Sequencing as opposed to 2x101 or 2x76 for Whole Exome Sequencing), the fragmentation conditions need to be changed to accommodate longer fragment lengths.

For the Enzymatic Fragmentation kits, length is dependent on three factors: Time, Temperature, and DNA input.

For Mechanical fragmentation, conditions (time, power, duty factor, and cycles per burst) should to be set to accommodate longer fragment lengths.

Yes, in addition to our Enzymatic Fragmentation kits, Twist also offers the following library preparation kits for use with Mechanical Fragmentation:

100875: Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples (includes enzymes and buffers)

101280: Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples (includes enzymes, buffers, and purification beads)

100876: Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples (includes enzymes and buffers)

101281:  Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples (includes enzymes, buffers, and purification beads)

Note for library preparation, you will also need adapters:

101307: Twist Universal Adapter System, 16 Samples

101308: Twist Universal Adapter System, 96 Samples – Plate A

101309: Twist Universal Adapter System, 96 Samples – Plate B

101310: Twist Universal Adapter System, 96 Samples – Plate C

101311: Twist Universal Adapter System, 96 Samples – Plate D

Assuming an input of 50 ng of DNA, the ratio of adapter to DNA is approximately 200:1.

Yes, Twist sells NGS kit components to allow for maximum flexibility.  You can obtain a complete list of orderable components by logging into your Twist Account.  You can order the following components:

 Library Prep Components      

• PN 100253 – TWIST LIBRARY PREP EF KIT 1, 16 SAMPLES    
• PN 100875 – TWIST LIBRARY PREP MF KIT 1, 16 SAMPLES   
• PN 100401 – TWIST LIBRARY PREP KIT 2, 16 SAMPLES                     
• PN 100572 – TWIST LIBRARY PREP EF KIT 1, 96 SAMPLES    
• PN 100876 – TWIST LIBRARY PREP MF KIT 1, 96 SAMPLES   
• PN 100573 – TWIST LIBRARY PREP KIT 2, 96 SAMPLES                     

Combinatorial Adapters        

• PN 100255 – 16 SAMPLES 

Unique Dual Indexed Adapters         

• PN 101307 – TWIST UNIVERSAL ADAPTER SYSTEM, 16 SAMPLES    
• PN 101308 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE A           
• PN 101309 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE B
• PN 101310 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE C
• PN 101311 – TWIST UNIVERSAL ADAPTER SYSTEM, 96 SAMPLES – PLATE D

Blockers         

• PN 100856 – TWIST UNIVERSAL BLOCKERS, 2 REACTIONS   
• PN 100578 – TWIST UNIVERSAL BLOCKERS, 12 REACTIONS 
• PN 100767 – TWIST UNIVERSAL BLOCKERS, 96 REACTIONS             

*Hybridization Reagents         

• PN 101263 – HYBRIDIZATION REAGENTS, 2 REACTIONS      
• PN 100930 – HYBRIDIZATION REAGENTS, 12 REACTIONS    
• PN 100982 – HYBRIDIZATION REAGENTS, 96 REACTIONS    

*Fast Hybridization Reagents  

• PN 101260 – FAST HYBRIDIZATION REAGENTS, 2 REACTIONS         
• PN 100964 – FAST HYBRIDIZATION REAGENTS, 12 REACTIONS       
• PN 100968 – FAST HYBRIDIZATION REAGENTS, 96 REACTIONS       

*Wash Buffers 

• PN 101264 – WASH BUFFERS, 2 REACTIONS 
• PN 100985 – WASH BUFFERS, 12 REACTIONS          
• PN 100846 – WASH BUFFERS, 96 REACTIONS          

*Fast Wash Buffers      

• PN 101261 – FAST WASH BUFFERS, 2 REACTIONS    
• PN 100971 – FAST WASH BUFFERS, 12 REACTIONS  
• PN 100972 – FAST WASH BUFFERS, 96 REACTIONS  

Purification and Binding Beads          

• PN 101262 – PURIFICATION AND STREPTAVIDIN BEADS, 2 REACTIONS      
• PN 100983 – PURIFICATION AND STREPTAVIDIN BEADS, 12 REACTIONS    
• PN 100984 – PURIFICATION AND STREPTAVIDIN BEADS, 96 REACTIONS   

*Note:  Standard Hybridization reagents should only be used with Standard Wash Buffers; conversely, Fast Hybridization reagents should only be used with Fast Wash Buffers.  Mixing Standard Hybridization and Fast Wash Buffers (and vice versa) will result in poor performance. 

Yes, Twist adapters are compatible with DNA fragments generated by either enzymatic or mechanical fragmentation.

Yes, in addition to our Mechanical Fragmentation kits, Twist also offers the following library preparation kits for use with Enzymatic Fragmentation:

100253: Twist Library Preparation Kit, Enzymatic Fragmentation, 16 Samples (includes enzymes and buffers)

101059: Twist Library Preparation Kit, Enzymatic Fragmentation, 16 Samples (includes enzymes, buffers, and purification beads)

100572: Twist Library Preparation Kit, Enzymatic Fragmentation, 96 Samples (includes enzymes and buffers)

101058:  Twist Library Preparation Kit, Enzymatic Fragmentation, 96 Samples (includes enzymes, buffers, and purification beads)

Note for library preparation, you will also need adapters:

101307: Twist Universal Adapter System, 16 Samples

101308: Twist Universal Adapter System, 96 Samples – Plate A

101309: Twist Universal Adapter System, 96 Samples – Plate B

101310: Twist Universal Adapter System, 96 Samples – Plate C

101311: Twist Universal Adapter System, 96 Samples – Plate D

Twist offers complete* kits for both Enzymatic Fragmentation and Mechanical Fragmentation - note these kits do not include an enrichment panel:

Enzymatic Fragmentation            

• PN 101897 – TWIST EF LIBRARY PREP + UDI AND STANDARD HYB, 16x2 REACTIONS          
• PN 101896 – TWIST EF LIBRARY PREP + UDI AND FAST HYB, 16x2 REACTIONS     
• PN 101901 – TWIST EF LIBRARY PREP + UDI AND STANDARD HYB, 96x12 REACTIONS       
• PN 101900 – TWIST EF LIBRARY PREP + UDI AND FAST HYB, 96x12 REACTIONS    

Mechanical Fragmentation        

• PN 101899 – TWIST MF LIBRARY PREP + UDI AND STANDARD HYB, 16x2 REACTIONS       
• PN 101898 – TWIST MF LIBRARY PREP + UDI AND FAST HYB, 16x2 REACTIONS    
• PN 101903 – TWIST MF LIBRARY PREP + UDI AND STANDARD HYB, 96x12 REACTIONS     
• PN 101902 – TWIST MF LIBRARY PREP + UDI AND FAST HYB, 96x12 REACTIONS

* Note: you must purchase KAPA® HiFi HotStart ReadyMix (Kapa Biosystems®, #KK2601) or its equivalent to generate and amplify libraries.

The recommended average target fragment size is 200 bp; this will result in an average library size of approximately 350 bp; however, you should adjust your insert size according to your sequencing read lengths.