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Methylated UMI Adapters
- Are the methylated UMIs compatible with gDNA as well as cfDNA?
- Do you have guidance on how to process sequencing data generated with the methylated UMIs?
- Are the methylated UMIs compatible with NEB EM-seq?
- How many bases are the UMI sequences?
- Are the UMI sequences available?
- What is the adapter structure of the methylated UMIs?
Are the methylated UMIs compatible with gDNA as well as cfDNA?
Yes, however increases in library complexity may not be as dramatic as those seen with low input cfDNA.
Still have questions? Contact Us
Do you have guidance on how to process sequencing data generated with the methylated UMIs?
Yes, bioinformatic processing steps are outlined in the product sheet.
Still have questions? Contact Us
Are the methylated UMIs compatible with NEB EM-seq?
Yes, Twist Methylated UMIs can be used as a drop in replacement during the library preparation step.
Still have questions? Contact Us
How many bases are the UMI sequences?
5 actionable bases and 2 skip bases.
Still have questions? Contact Us
Are the UMI sequences available?
Yes, upon request after purchase, Twist Technical Support can provide the UMI sequences. Email customersupport@twistbioscience.com for support.
Still have questions? Contact Us
What is the adapter structure of the methylated UMIs?
Twist Methylated UMI Adapters are composed of 32 discrete pairs of adapters with in-line UMI sequences, meaning they are present just after the sequencing primer binding site.
Still have questions? Contact Us
Are the methylated UMIs compatible with gDNA as well as cfDNA?
Yes, however increases in library complexity may not be as dramatic as those seen with low input cfDNA.
Still have questions? Contact Us
Do you have guidance on how to process sequencing data generated with the methylated UMIs?
Yes, bioinformatic processing steps are outlined in the product sheet.
Still have questions? Contact Us
Are the methylated UMIs compatible with NEB EM-seq?
Yes, Twist Methylated UMIs can be used as a drop in replacement during the library preparation step.
Still have questions? Contact Us
How many bases are the UMI sequences?
5 actionable bases and 2 skip bases.
Still have questions? Contact Us
Are the UMI sequences available?
Yes, upon request after purchase, Twist Technical Support can provide the UMI sequences. Email customersupport@twistbioscience.com for support.
Still have questions? Contact Us
What is the adapter structure of the methylated UMIs?
Twist Methylated UMI Adapters are composed of 32 discrete pairs of adapters with in-line UMI sequences, meaning they are present just after the sequencing primer binding site.
Still have questions? Contact Us