How do I set up 8-plex Target Enrichment with libraries made from different sample mass inputs?
The maximum recommended library yield into target enrichment is 12.8 μg. When pooling libraries from variable starting mass inputs, each library should have the same input mass added to the pool for uniform sequencing allocation post-capture.
To calculate that mass, multiply the highest mass input by 80x. For example:
- With an 8-plex where the highest sample mass input is 20 ng, pool 1,600 ng from each library for a total of 12.8 μg.
- With an 8-plex where the highest sample input mass is 10 ng, pool 800 ng from each library for a total of 6.4 μg.
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