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From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
Cell-free protein expression can accelerate discovery in synbio. Gene fragments are an optimal substrate for cell-free protein production
A cornerstone of synthetic biology is designing DNA sequences and testing the characteristics and functions of the resultant proteins. Here, Twist Gene Fragments were used as DNA input of a cell-free protein expression study using multiple commercially available in vitro protein production kits. The results demonstrate that Twist Gene Fragments enable high-throughput protein expression and functional testing within a single day.
Covered in this Application Note
Design of Twist Gene Fragments for cell-free protein expression
Cell-free expression of the BamHI restriction enzyme
Analysis of cell-free expressed BamHI activity compared to purified BamHI.
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