Library construction for Next Generation Sequencing (NGS) using formalin-fixed paraffin-embedded (FFPE) samples offers unique challenges in acquiring high-quality sequencing data due to wide distribution of sample quality. Differences in formalin fixation methods, storage conditions, and age lead to cross-linked and/or degraded nucleic acids and inconsistent extraction yields. Therefore, FFPE extraction and library construction methods must be carefully considered for target enrichment applications. In collaboration, Covaris and Twist Bioscience demonstrate a complete library generates read-to-sequence multiplexed libraries directly from FFPE tissue of various qualities.