The use of CRISPR screening technology enables the interrogation of gene function at virtually any scale, especially in pooled screening applications. While genome-wide CRISPR screen libraries are easily accessible off-the-shelf, their lack of design flexibility means it is difficult to cost-effectively address specific research questions, thus limiting the applicability of these libraries. Twist Bioscience’s custom CRISPR guide libraries give you the flexibility to tailor your sgRNA library to your species, strain, and research question, all with the confidence that guides in your library will be precisely synthesized and equally represented.