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Processing Sequencing Data Utilizing Twist Unique Molecular Identifier (UMI) Adapter System
This guide provides recommendations for processing Illumina sequencing data from samples prepared with the Twist UMI Adapter system and for calling consensus reads from UMI families. Libraries prepared with the Twist UMI Adapter system contain a 5 base pair unique molecular identifier and a 10 base pair unique dual index, as well as standard p5 and p7 adapters.
