Twist High Throughput Antibody Production is a gene-to-protein workflow that can produce tens to thousands of diverse antibodies in small-scale quantities for discovery and screening purposes. This document will guide you through the process of preparing your sequences and placing an order within Twist’s ordering platform.

The workflow starts with choosing VHH-Fc or conventional IgG antibody expression and submission of the desired antibody sequences into Twist’s intuitive ecommerce platform. Bioinformatic screening ensures that potential sequence liabilities that may affect antibody expression are mitigated. Once the order is received, Twist will synthesize and clone antibody genes into a vector of your choosing (either a custom vector or one of Twist’s IgG scaffold vectors). Twist antibody engineers will then express, affinity purify, and quality control the requested antibodies.