This poster presents a case study of a 28,000-head cow-calf operation transitioning from array-based genotyping to sequencing-based genotyping with Curio Genomics and Twist Bioscience. The study demonstrates the feasibility and accuracy of using Twist's HTP self-normalizing FlexPrep library prep and multiplex capture to target genomic regions previously captured by 70k bovine arrays. Results show 99%+ concordance between array and targeted sequencing data, providing confidence to complete the switch and improve breeding program efficiency.

Key Learnings:

• Sequencing-based genotyping offers a viable and accurate alternative to traditional array-based methods, with high concordance rates.

• A minimum of 30 nanograms of DNA input consistently provides sufficient coverage (20x+) and genotype accuracy (99%+) for targeted sequencing.

• The CURIO agrigenomics data analysis platform allows for simultaneous visualization and comparison of both microarray and NGS-based genotype results.