Next Generation Sequencing technology has been a driving force for reducing sequencing costs and enhancing genome technology, allowing investigators and clinicians the opportunity to better delineate an individuals’ genetic makeup. For facilities providing genetic services, maintaining the balance of generating high quality data at the lowest cost is a continuous effort requiring recurrent evaluation and optimization of protocols. In preparation for higher throughput of exome sequencing using the NovaSeq, [CIDR] evaluated uniformity, coverage and cost of sequencing to achieve 90% on target at 10x, 20x and 30x depth across four different exome capture products.