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Targeted sequencing allows for high-resolution characterization of gene regions at a scale and cost that is more accessible than whole genome sequencing. Here we describe a method to leverage Twist Bioscience target enrichment workflow for gene panels sequenced with HiFi reads. This poster presents the content and the performance of two Twist Alliance panels, pharmacogenomics panel with the Twist Human Mitochondrial Panel and a nearly 400-gene panel of challenging “dark genes” relevant to human health.