Twist Bioscience has expanded a range of next generation library offerings which include CRISPR single guide RNA (sgRNA) and dual-guide (dgRNA) libraries, Synonymous Codon (SynCodon) libraries, and Paired T-cell receptor (TCR) and Chimeric Antigen Receptor T-cell (CAR-T) libraries. Utilizing Twist Bioscience’s silicon DNA synthesis platform, precise mutations are individually synthesized and introduced via high quality oligo pools and gene fragments. Coupled with our molecular biology expertise in gene synthesis and DNA assembly, Twist Bioscience can build fulllength, highly diverse, and precise libraries to maximize your screening capabilities.

CRISPR sgRNA and dgRNA, libraries are an efficient tool for high throughput gene editing and knockout of molecular targets. By leveraging the specificity of the CRISPR gene editing technology, researchers can combine their designed gRNAs and lentiviral plasmid, to create a ready-to-transduce library product. In recent literature, dgRNA libraries have become increasingly popular. The use of two gRNAs to target a single or multiple genes can help create a higher frequency and a higher specificity of mutations. With the use of efficient single stranded DNA isolation techniques, Twist can generate dgRNAs with >90% matching between two sets of gRNAs.

SynCodon libraries allow researchers to explore an emerging space in enzyme engineering. These libraries involve created mutations of a synonymous codon of each amino acid in a protein sequence. Codon optimization can help improve protein yields, binding affinity, stability, and expression of proteins. With the use of SynCodon Libraries customers can determine the most optimal synonymous codon usage for a given protein.

TCRs and Chimeric Antigen Receptor T-cell (CAR-T) libraries when assembled combinatorially can help screen a large scale of module sets within both library types. However with new long read sequencing technologies, customers have higher confidence in their alphas and beta pairing for TCR libraries and in conjunction antibody binding (scFv, Fab, VHH, scFab), hinge, transmembrane and signaling domain pairings for CAR-T libraries and therefore have specified designs for each module type. Twist utilizes a patented DNA assembly technology to effectively assemble unique combinations of domains in a high throughput approach to make downstream screening as precise and efficient as possible.

Synthetic libraries are based on computational in silicon design and use synthetic DNA fragments to introduce diversity. Using Twist’s unique silicon-based DNA synthesis platform, we can generate individualized modular domains for synthetic library production. These modules are constructed with novel DNA assembly technologies to provide diversities up to ~10⁴ - 10¹⁰