Optimized Library Preparation Kit and Workflow for Improving cfDNA Sequencing Sensitivity

Next-Generation Sequencing (NGS) of cell-free DNA (cfDNA) has emerged as a promising strategy for early detection,diagnosis, and monitoring of cancer progression. In this process, cfDNA is extracted from a patient’s blood and undergoes library preparation prior to NGS. The challenge with cfDNA library preparation is reliably capturing and converting all the fragmented DNA, especially when present in low concentrations within biological samples. Attaining low variant detection thresholds and variant calling confidence demands high-performance NGS libraries and targeted sequencing protocols.

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Workflow leveraging the Twist cfDNA Library Preparation Kit and an optimized target enrichment protocol to maximize the conversion

Demonstrated by increasing target coverage you can increase detection sensitivity at low variant allele frequencies

The workflow improves detection of duplex-molecule families relative to comparable workflows

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Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Optimized Library Preparation Kit and Workflow for Improving cfDNA Sequencing Sensitivity

Related Resources

cfDNA Library Prep Kit Datasheet

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