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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
TAO: Twist Antibody Optimization of an Anti-PD-1 Antibody
Presented at PEGS 2019: The TAO platform enables high-throughput, high-quality characterization of high-affinity, functional antibodies
We used the Twist Antibody Optimization platform (TAO), a custom algorithm to explore the CDR mutation space from reference sequence as well as the closest germline. The platform was applied to optimize a low-affinity antibody to human PD-1. Several variants demonstrated 50-100x improved monovalent binding affinities, comparable to those from commercial antiPD-1 antibodies, while maintaining on-cell binding and retaining the original antibody-binding epitope.
