Multiple highly diverse synthetic antibody libraries have been generated using Twist’s precise DNA synthesis technology to empower the antibody discovery process. A few of these “Library of Libraries” include the Structural scFv Library, AI Hypermutated scFv Library and hCamel Zero VHH Library. By leveraging these synthetic libraries, a wide range of high-affinity antibody candidates have been identified across multiple protein targets. 

In this project, we demonstrate the validation process of constructed synthetic antibody libraries. Phage display was used to pan the libraries against biotinylated protein targets and ELISA binding assays were performed to screen for unique candidates. A high- throughput protein purification process allows candidates to be reformatted into VHH-Fc or IgG, which are then characterized through SPR.