Gene Fragment Cloning Guidelines

Recommendations for cloning and amplifying Twist Gene Fragments.

Twist Bioscience’s Gene Fragments are double-stranded DNA, 300 to 5000 base pairs in length. These high-quality fragments come with unique 5’ and 3’ adapter sequences. These guidelines offer recommendations for cloning Twist fragments, as well as considerations for designing Gene Fragment sequences that are compatible with downstream cloning or amplification methods.

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Gene Fragment Cloning Guidelines

Related Resources

Enzyme identification and engineering for novel industrial enzymes

Gene Fragments

From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments