Library Preparation EF 2.0 with Enzymatic Fragmentation and Combinatorial Dual Indices

For use with the Twist NGS Workflow

This Twist Library Preparation Kit provides the reagents needed to prepare genomic DNA (gDNA) libraries using enzymatic gDNA fragmentation and Y-shaped adapters. This manual details the steps for generating the amplified, indexed libraries needed for downstream target enrichment and sequencing on Illumina next generation sequencing (NGS) systems. This library preparation protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.

TWT: EF 2.0 fragmentation master mix preparation and addition to sample

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Library Preparation EF 2.0 with Enzymatic Fragmentation and Combinatorial Dual Indices

Related Resources

Optimizing the Performance of the Twist Targeted Methylation Sequencing Workflow

Minimizing Off-Target Capture in Targeted Methyl-Seq Through Panel Design & Enhancer Reagents

Capabilities of the Twist NGS Methylation Detection System