Library Preparation Protocol - Mechanical Fragmentation and Combinatorial Dual Indices

A comprehensive protocol for genomic DNA preparation for downstream target enrichment and sequencing on Illumina systems

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Library Preparation Protocol - Mechanical Fragmentation and Combinatorial Dual Indices

This protocol is for the preparation of genomic DNA (gDNA) libraries using mechanically fragmented DNA and Y-shaped adapters. This manual details the steps for generating the amplified, indexed libraries needed for downstream target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. This library preparation protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Library Preparation Protocol - Mechanical Fragmentation and Combinatorial Dual Indices

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