NEB Next Enzymatic Methyl-seq Library Preparation Protocol

For use with the Twist NGS Methylation Detection System

Factors beyond the genomic DNA sequence, including the methylation of adenines and cytosines, influence cellular functions and health. These epigenetic modifications regulate gene expression in a cell type-specific manner. Cytosine methylation is found in common genomic sequences called CpG sites, and methylation has been linked to several kinds of cancers and genetic disorders. Being able to accurately detect methylation patterns is not only important for understanding many biological processes in health and disease, but also for diagnosing cancer.

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

NEB Next Enzymatic Methyl-seq Library Preparation Protocol

Related Resources

Highly Sensitive Methylation Detection Using Enzymatic Methyl-seq and Twist Target Enrichment

NGS Methylation Detection System

Targeted Methylation Sequencing