One-Pot Hybridization Reaction Protocol

This document outlines an alternative hybridization reaction protocol that replaces the “Hybridize Capture Probes with Pools” step for Twist Standard Hyb v1, Standard Hyb v2, and FlexPrep Target Enrichment workflows. It is designed to limit the number of transfer steps required for the setup of capture reactions while maintaining high performance.

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

One-Pot Hybridization Reaction Protocol

Related Resources

Twist Library Preparation EF Kit 1 (SDS-001072)

Hybrid Capture Sequencing is More Robust than Amplicon Sequencing for Viral Surveillance

SARS-CoV-2 NGS Assay