This Trinity Freestyle Fast Hybridization protocol details the steps for performing target enrichment and sequencing on Twist indexed libraries generated with the Twist UDI Primers for Trinity Freestyle. The Twist for Element, Trinity Freestyle Fast Hybridization Kit enables rapid enrichment of libraries generated from Twist's most commonly used library preparation kits, such as Enzymatic Fragmentation 2.0 and FlexPrep UHT kits. The resulting enriched DNA library can be sequenced on Element next-generation sequencing (NGS) systems using the Trinity Freestyle sequencing workflow. This protocol should only be performed with the reagents specified or their equivalents.