Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
This Twist Whole Genome Sequencing Workflow details all the necessary steps for generating PCR-free indexed libraries for sequencing on Illumina next-generation sequencing (NGS) systems. The workflow prepares genomic DNA (gDNA) libraries using mechanically fragmented DNA and full-length Y-shaped adapters. This library preparation workflow has been optimized for use with the reagents specified and should only be performed with them or their equivalents.