Twist Oligo Pool Amplification Guidelines

Recommended reagents and protocol for PCR amplification of Twist oligo pools.

Twist uses phosphoramidite chemistry for oligo synthesis. Compared to conventional column-based synthesizers, our synthesis platform miniaturizes the synthesis process, which reduces reaction volumes and increases throughput. Because the resulting oligos are single-stranded and present at femtomole levels, they may need to be converted to double-stranded DNA or amplified for certain applications via PCR. PCR amplification also enriches the amounts of full-length oligo sequences.

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Twist Oligo Pool Amplification Guidelines

Related Resources

Scalable and combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted seq

Drugging the undruggable with protein interference

Multiple Focused CRISPR Libraries from a Single Oligo Pool