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Twist RNA Library Preparation with the UMI Adapter System
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This Twist RNA Library Preparation Protocol details all the necessary steps to build sequencing libraries for target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. The steps include converting RNA to double-stranded cDNA and preparing cDNA libraries using the Twist UMI Adapter System for sequencing. The Twist UMI Adapter System consists of Twist UMI Adapters and Twist Unique Dual Indexed (UDI) Primers. This protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.
