This protocol is for the generation of custom target-enriched DNA libraries with the Twist Custom Panel EF Workflow for sequencing on Illumina next-generation sequencing (NGS) systems. This manual describes all the steps required to construct libraries and perform single or multiplex hybridization reactions with Twist Custom Panels. Though optimized for use with Twist Complete Reagents EF for Custom Panels, the protocol described here can also be used with other library preparation methods. 

Troubleshooting with Twist: Standard hybridization capture probes with pools reaction setup

Troubleshooting with Twist: Twist Target Enrichment Fast Hybridization Protocol

Troubleshooting with Twist:(StdHybv1) Transfer of the hyb rxn to washed streptavidin beads