The Twist FlexPrep UHT Library Preparation and Target Enrichment Workflows are designed to address these limitations by introducing two technologies: normalization-by-ligation and inline read barcodes. These two technologies offer a solution to reduce the burden of DNA normalization and reaction footprint by generating 12 libraries in a single well. This datasheet describes general performance expectations of various library preparation parameters when using these workflows and details the versatility of these kits. Additionally, we provide guidance for achieving desirable performance from these workflows.