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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Efficient, exon-aware RNA capture for gene expression and novel fusions
Twist’s new RNA Exome panel uses a novel exon-aware design strategy to target known protein-coding isoforms and enable the discovery of novel fusion genes. We evaluated panel performance, showing relative transcript abundances are preserved after hybrid capture. We also show gains in sequencing efficiency and demonstrate the ability to capture novel structural variants, such as RNA fusions common in cancers. We discuss our bioinformatic approach to evaluate capture performance for RNA and challenges in the analysis of RNA-seq experiments.
Covered in this Technical Note
Learn about the exon-aware design strategy.
Understand the design strategy of Twist RNA Exome.
Evaluate targeted RNA sequencing performance.
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