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Highly Sensitive Methylation Detection Using Enzymatic Methyl-seq and Twist Target Enrichment
Discover how enzymatic methyl-seq and Twist Target Enrichment converge to provide unparalleled performance in methylation detection.
DNA methylation plays an important role in cell growth by regulating gene expression. In some cases, aberrant cytosine methylation can lead to carcinogenic changes in gene expression. Historically, these modifications have been hard to identify in patient samples. Although next-generation sequencing (NGS) of DNA treated with sodium bisulfite has provided an efficient means for the detection of methylated cytosines, the chemical conversion process is damaging, leading to GC biased coverage and poor complexity. Such protocols are therefore limited in their sensitivity.
Covered in this Technical Note
A comparison between library preparation methods using bisulfite and enzymatic conversion
How to use controls in the Twist Targeted Methylation Sequencing Workflow
The performance of the Twist Targeted Methylation Sequencing Workflow across target region sizes, GC contents
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