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Hybrid Capture Sequencing is More Robust than Amplicon Sequencing for Viral Surveillance
Our ability to intercept and combat new pandemic strains is contingent on robust and accurate technologies for viral genome sequencing. A common method for viral genome sequencing employs the polymerase chain reaction (PCR). This method is not robust over time when dealing with a rapidly mutating organism, as mutations can accumulate in amplicon primer binding regions, leading to drop-outs. Here we show that target capture sequencing is a robust alternative for pandemic strain surveillance that does not present dropouts in rapidly mutating strains
Covered in this Technical Note
How amplicon sequencing can cause dropouts when sequencing rapidly mutating disease strains
How Target capture is robust to dropouts, leading to accurate sequencing and surveillance of viruses like SARS-CoV-2
Examples of robust target capture on clinical samples that show dropouts with amplicon sequencing
