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Twist Tips: Meeting Minimum Length Requirements for Genes in Custom Vectors
Two options for ensuring successful synthesis of genes under 300bp in length
When you submit a sequence for synthesis, the scoring algorithm checks the sequence to determine whether it can be synthesized, and one of the first things it checks is whether the sequence meets the minimum length requirement of 300 bp. Use this document to help you design your Genes when they do not meet the minimum length requirement.
Covered in this Technical Note
Guidelines for adding stuffer sequence into a gene that is less than 300bp in length.
Guidelines for adding vector sequence into a gene that is less than 300bp in length
How to move
the insertion point in a custom vector
