Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens

Dr. Stephen Pettitt explores the limits of genome-wide targeted mutagenesis screens and the genetic basis of gene resistance

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Presented by

Stephen Pettitt, PhD

Institute of Cancer Research, UK

Covered in this Webinar

Learn about the role of Poly-ADP Ribose Polymerase (PARP) in DNA Repair.

How structure-function relationships of PARP1 mutants inform treatment of cancer patients with these drugs.

How to use genome-wide CRISPR screening for mutant discovery.

How to create a highly diverse, sgRNA library from Twist Bioscience for targeted, subtle mutations.

Explore the limits of genome-wide targeted mutagenesis screens and the genetic basis of gene resistance with Dr. Stephen Pettitt, Staff Scientist, Institute of Cancer Research, UK.

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Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens

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