CRISPRgate addresses these issues by using two specific sgRNAs to both repress and cleave the target gene within the same cell, significantly increasing the efficiency and reproducibility of genetic screens.In this webinar, we collaborated with BiteSize Bio and Philipp Rathert, PhD, Lecturer from tUniversität Stuttgart to explore how researchers have successfully used Twist’s cloned 300mer oligo pools, to implement CRISPRgate, resulting in improved depletion efficiency, reduced sgRNA performance variance, and more consistent phenotypic effects. Whether you're aiming to enhance your gene editing projects or seeking to understand the future of genetic screening, this webinar will provide you with valuable insights and practical applications.