A major challenge in controlling the COVID-19 pandemic is the high false-negative rate of the commonly used RT-PCR methods, particularly in samples with low viral loads. We implemented a three-step approach for SARS-CoV-2 detection and quantification that employs reverse transcription, targeted cDNA preamplification, and nano-scale qPCR based on a microfluidic system. 
Covered in this webinar:
Using Twist SARS-CoV-2 synthetic RNA and clinical samples, we demonstrate that preamplification step enhances the LoD by 1000-fold, enabling detection at 1 copy/uL and reducing false-negative rate of detection. 
How this approach can robustly enhance the sensitivity of detecting SARS-CoV-2 and potentially other viral infections.